| PLGA electrostatic spinning film, combined with advantages of electrospinning and highmolecular polymer, not only have high porosity and high-oxygen transmission rate, but alsohave good biocompatibility，good film-forming ability and autoproteolysis. Its propertiesallow the wound liquid oozing in time, and ensure that the wound wettability. It can protecttissue against the bacteria, and then create a favorable environment for tissue growth andhealing. It can automatically degrade without leaving foreign matter and toxic andside-effects.This paper focused on the study of the toxicity of PLGA electrostatic spinning film,effects of anti-adhesion, and rats’ in vivo tests, and the results are showed as follows:1．The cell test of PLGA membrane(1) Through co-culture the PLGA membrane with IEC-6and NIH3T3, detected byCCK-8test, the results show that the PLGA membrane is not of cell toxicity.(2) In the MTT assay, two of cell lines IEC-6, NIH3T3were incubated in the differentPLGA film by a series of treatments against the control, the PLGA film does not adhered bythe cell easily.(3) The two of cell lines IEC-6, NIH3T3were incubated at different membranes andtaking pictures assisted by the fluorescent dye. It could be observed that thenumber of thecells adhered to the PLGA membranes were less than that of the control group.(4) Co-cultured in the PLGA membrane degradation solution with IEC-6and NIH3T3cells, respectively, the morphology observed by scanning electron microscope shows thatthere is no significant difference between treatment and control.2．In vivo test of PLGA membrane anti-adhesion(1) The animal models were established. Complications and adhesions were observed.The results showed that the PLGA electrospun membrane significantly reduced the risk ofcomplications and adhesion compared with the control.(2) There is not significantly difference between the number of the red blood cell andwhite blood cell, indicating that the PLGA membrane has no apparent toxicity and irritation.(3) Analyzing the prothrombin time, the activated partial thromboplastin time and theprothrombin coagulating time, all of these three indicators are within the normal range. Thereis no coagulating phenomenon. It shows that the PLGA membrane does not affect thecoagulation of the blood in rats.(4) During the acute phase, the SOD value of the negative group was significantly lower than the normal group. The SOD value of the160μm of the PLGA membrane group wassignificantly higher than that of the negative group (P <0.05). The others had no significantchange.The SOD of Sub-acute rat of peritoneal lavage fluid: the SOD value of negative group,positive group, PLGA membrane group, the SOD value of the60μm group were significantlylower than that of the normal group (P <0.05). The others had no significant difference.The SOD of chronic rat of peritoneal lavage fluid: the SOD value of the negative groupwas significantly lower than that of the normal group (P <0.01). The SOD value of thepositive was significantly lower than that of the normal group (P <0.05). The SOD value ofthe positive group and the160μm PLGA membrane group were significantly higher than thatof negative group (P <0.01).(5) The MDA of the acute phase rat of peritoneal lavage fluid: the MDA of the negativegroup and positive group were significantly higher than that of the normal group (P <0.01).The MDA values of the160μm of PLGA film and the60μm PLGA membrane group weresignificantly higher than that of the normal group (P <0.05).The MDA of sub-acute rat peritoneal lavage fluid: the MDA of the negative group wassignificantly higher than that of the normal group (P <0.05). Other groups have no significantchanges.The MDA of Chronic rat peritoneal lavage fluid: The MDA of negative group issignificantly higher than that of normal group (P <0.05). The MDA of the positive group andthe160μm PLGA membrane group is significantly lower than that of the negative group (P<0.01).3. ConclusionThe results of the PLGA electrostatic spinning film show that，it has no cytotoxicity, andcan effectively prevent the cell adhering on the membrane. By the in vivo test, thecomplications and adhesion can significantly reduce by applying the PLGA electrostaticspinning films. The results of routine blood and coagulation index test of rats show that thereis no immunogenicity and no effect on rats’ blood coagulation. Compared with the SOD andthe MDA values of acute phase, subacute, and chronic phase of peritoneal lavage fluid of rats,the PLGA electrostatic spinning film contribute to the capacity of rat’s own free radicalscavenging recovery, significant improve mucosal damage, speed up wound healing. |
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