Studies On The Morphology And Morphogenesis Of Gonostomum Namibiense(Ciliophora:Hypotrichida)

Gonostomum namibiense have been the good material in cell and molecular biology for its special evolutionary status and the single-celled eukaryotes. Its cytoskeleton, physiological mechanisms, metabolic regulation, and other aspects of the system has been the hotspot in evolution of life science research. The cell come from the alkaline soils around Arabian desert, Ciliature dorsal and ventral cortex were stained to observe Infraciliature over Protargol staining, and then explore its Infraciliature morphological similarities and differences with others, and speculated that the cilia schemata rebuild; using fluorescent paclitaxel on cortical microtubular organelle to show the cortical microtubular cilia and its associated microtubule distribution, using scanning electron microscopy to observed some structural details. While taking advantage of the results to supplement deficiencies and validation, the corresponding results and conclusions are discussed below:(1) Gonostomum namibiense cortex CiliatureGonostomum namibiense size in130-200×20-25μm, blunt tip and tail slender, cortex covered with pigment granules, transparent and gray. Some cells have vesicular, ranging from the number. Macronucleus with2-3accompanied by micronucleus, its number between2-5. Gonostomum namibiense cortex Ciliature include Adoral zone of membranelles, Undulating Membrane, frontal cirri, Buccal cirri, frontalterminal cirri, ventral cirri, transverse cirri, marginal cirri, caudal cirri, dorsal cirrus and other components. Gonostomum namibiense cortical microtubules class structure is mainly concentrated in the ventral, dorsal cortical microtubules class structure is not well developed.(2) Gonostomum namibiense morphogenesisThe occurrence of cortical cilia around the buccal region begins with the proliferation of primordia to form thin wire-like structure, eventually becoming the mouth, with the development of oral primordia, the right of oral primordia lies six banded structure. From left to right Ⅰ,Ⅱ, Ⅲ,Ⅳ,Ⅴ,Ⅵ.Ⅰ,Ⅱ,Ⅲ front primordia makes frontal cirri. frontoterminal cirri develops from VI, transverse cirri come from Ⅴand Ⅵ. Marginal cirri morphogenesis began with the old cirri collapse, followed by in situ proliferation of a new intensive margin cirri primordia, and began to migrate across the cell. New caudal cirri come from dorsal cirrus..Gonostomum namibiense morphogenesis particular characteristics reflected in:First, the differences of cortex ciliatures morphogenesis. Second, the fate of old cirri and primordium. In Gonostomum namibiense morphogenesis process, the number of Macronucleus are relatively constant, but the small number of micronucleus are fluctuant. It may associate with reproduction regulatory.