Study On The Nutritional Values And Physiological Function Of Scapharca Subcrenata Hydrolysates

Scapharca subcrenata is a significant economic shellfish widely bred in littoral of China. People like it for its peculiarity of high contents of protein, low fat content and its remedy effects. Scapharca subcrenata is object of study in this dissertation. In order to offer foundation for preparing peptide from Scapharca subcrenata,the aim of this experiment was to study the effects of hydrolyzing conditions on the hydrolysates of Scapharca subcrenata, the optimal technological of hydrolyzing Scapharca subcrenata with protease, and the characterization of peptide. The main results were as follows:1. The effects of hydrolyzing conditions on the hydrolysates of Scapharca subcrenata. Enzyme quantity, temperature, pH and hydrolysis time were selected as four orthogonal factors. The results by using neutral protease to hydrolyze were as follows:enzyme quantity of0.8%, temperature50℃, pH7.5and time7h. Otherwise,the results by using papain were as follows:enzyme quantity of1.0%, temperature45℃, pH7.5and time8h.2. Study on the denaturation level in different treatment and the effect of enzymatic hydrolysis. The result show that denaturation level rose while disadvantageous enzymatic hydrolyzing, the DH was reducing.3. Used by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the result with molecular weight demonstrate show that lots of peptides from Scapharca subcrenata hydrolysate was about14400Da.4. The diets were prepared with the crude Scapharca subcrenata meat or two kinds of protease hydrolysates and compared with casein. In order to study the nutritional values and physiological function of Scapharca subcrenata hydrolysates used pair fed method. The findings in antitumor test shown that neutral protease hydrolysates groups could improved the digestibility and protein efficiency ratio compared with crude group.On aspect of blood lipids, neutral protease hydrolysates group diet could depress the serum cholesterol (TC), triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C), while also can increase high-density lipoprotein cholesterol (HDL-C). So hydrolysis of Sinonovacula Constricta Lamarck obtain peptides had functions on blood lipids metabolism.On the antioxidation aspect, neutral protease hydrolysates group diet can improve the activities of SOD and GSH-Px, while also can reduce the concentration of MDA in serum and liver.5. The70mice were randomly divided into seven groups, the normal control group was fed by common feeds stuffs, while other groups fed by high-fat diet. Normal and model control groups were offered by corresponding volume of distilled water, two positive drug groups were offered by Vitam in C with20mg/(kg’d) and simvastatin with0.5mg/(kg’d) respectively. Large, medium and small dose groups were taken medicine by neutral protease hydrolysates with1500,1000and500mg/(kg·d) dose.The serum and tissue of rats in treatment groups was determined6weeks after treatment.The protease hydrolysates of Scapharca subcrenata had potent effects on regulating blood lipids in hyperlipidemic rats. Protease hydrolysates could significantly reduce the TC,TG,LDL-C and increase HDL-C level in serum.The activity of SOD and GSH-Px had raised, content of MDA had degraded, which proved that protease hydrolysates of Scapharca subcrenata regulated the antioxidation enzyme activity and then reduced the lipid peroxidization.