Preliminary Analysis Of Arabidopsis Temperature Sensitive Mutant S7-11

This paper chooses Arabidopsis thaliana as the research object in order to investigate the effect of environment temperature alternatives and other factors on the direction of root growth. A temperature sensitive mutant s7-11has been isolated. Through the analysis of research, Hypocotyl length of mutant s7-11get much longer than wild type and left deflection angle of roots become larger when the temperature is increasing(17℃,22℃,27℃). Meanwhile s7-11shows the angle of leaf epinasty significantly higher than the wild type, and exhibits early flowering. After comparing root deflection of s7-11and F1, which was hybridized by s7-11and wild-type, the root of Fl generation indicates left deflection but with small angel. After exogenous of auxin, the deflection angles of their roots are growing bigger by the influence of auxin.When applied the exogenous cytokinin, the root left deflection angle of s7-11changed, besides, the change of angle decreased with the increasing concentration. Using sodium chloride will not only change root growing direction of the s7-11from left to right but also change the root direction of the wild-type. It proves the mutant root deflection relate to auxin,cytokinin and sodium chloride indeed. By studying the relationship between transformation of root deflected direction and hormone as well as salt,this research indicates the connection of tempurature,hormone and salt on influencing root growth direction. It also provides a primary evidence of researching the connection between tempurature signal pathway and other signal pathways. In conclusion, the facts of external environment such as temperature, hormone or salt have an important influence on root growth and development.