Study On The Population Differentiation And Genetic Diversity Of Drosicha Corpulenta Populations On Different Hosts And Geography

In this paper ,the genetic diversity and population differentiation of Drosicha corpulenta on sixteen different hosts collected from Baoding,Shijiazhuang and Handan in Hebei were analyzed by random amplified polymorphic DNA (RAPD) technique. The genetic diversity parameters of the populations were analyzed with software such as the Popstream32. And the cluster graph of different populations was made according to the genetic similarity coefficient. The main results and conclusions are as follow.1、The high quality genome DNA can be acquired with the improved method of CTAB, which meets the demand of the following experiences.2、Genetic diversity of Drosicha corpulenta populations on different hosts and geographyA total of 41 Polymorphic locis were obtained from 4 effective primers and the percentage of population polymorphic loci was 100%. It showed that the genetic diversity of Drosicha corpulenta populations were high.The Shannon’s information index(I) of the 16 populations ranges between 0.1708 to 0.2967 and the average H was 0.2306. The I of HS population was the highest, while the I of HW population was the lowest. The Nei ’s genetic diversity (H) ranges from 0.1101 to 0.1991 and the average I was 0.1511. It was the highest in the HS population, while the lowest in the BBT population. It indicated the genetic diversity of Drosicha corpulenta varied with different populations.3、Population differentiation of Drosicha corpulenta on different hosts and geographyThe genetic distance index was 0.0701～0.4360, and the average value was 0.2395. The HF population was similar to the HC population mostly, which genetic distance index was 0.0701. The longest genetic distance(0.4360) was between the SZ population and the HM population. This showed the genetic variation among populations were quite high.QCluster analysis showed that the majority of individuals of the same geographic host populations could be clustered into one class. This indicated the individual genetic similarity was high, whicn has guaranteed the heredity stability in the identical population. Each population on the same host was clustered respectively at first. When similarity coefficient was 0.642, the 16 Drosicha corpulenta populations could be separated into 6 groups. The first group included BB,BZ,BBT,BY,SB,SC,HC,HW, HF, HS,HM; the second group only included SX; the third group included HL and SZ;the forth group included HZ; the fifth group included HB. The above result indicated that the Drosicha corpulenta populations on different hosts existed distinctly population differentiation.The different geographic population(Baoding, Shijiazhuang, Handan)on hosts of Amygdalus persica f. pendula and Prunus ceraifera f. atropurpurea were clustered respectively at first, then the populations from Shijiazhuang and Baoding were clustered together, at last the population from Handan joinged in. It showed the different geographic population exited population differentiation and geographical distance effect on the genetic variation. But Drosicha corpulenta populations on different hosts and geographic regions have not existed apparent geographic cluster, suggesting that the host and geographic factors both effected the population differentiation of Drosicha corpulenta at the same time.