Effects Of Huangshu Enema On Mast Cells And The Substance P In Rats With Diarrhea-predominant Irritable Bowel Syndrome

AIM:To evaluate the effects of Huangshu Enema (HSE) on mast cells in ileocecaljunction and the substance P in serum and organization homogenate of the ileocecaljunction in rats with diarrhea-predominant irritable bowel syndrome (D-IBS).METHODS:Thirty-six female Sprague-Dawley rats were randomly divided into normalcontrol group (n=9), model control group (n=9), HSE treatment group (n=9) andDicetel treatment group (n=9). A rat model of D-IBS was created by intracolonicinstillation of acetic acid and restraint stress. Observe the change of the number ofmast cells in ileocecal junction of rat by Toluidine blue improved dyeingmethod.Test the content of substance P in serum and organization homogenate of theileocecal junction by ELISA.RESULTS:The number of mast cells in ileocecal junction was significantly higher in themodel control group than the normal control group (3.07±0.56vs1.47±0.26; t=7.787,P<0.01), The content of substance P in serum and organization homogenate of theileocecal junction were significantly increased in the model control group than thenormal control group (600.38±175.25vs181.12±40.03,194.01±35.54vs60.01±9.33;t=6.997, t=10.943, P<0.01); The number of mast cells in ileocecal junction and thecontent of substance P in serum and organization homogenate of the ileocecaljunction were significantly reduced in the HSE treatment group than the modelcontrol group (1.53±0.26vs3.07±0.56,237.86±19.75vs600.38±175.25,88.29±5.51vs194.01±35.54; P<0.01); The number of mast cells in ileocecal junction and thecontent of substance P in serum had no significant difference between the HSEtreatment group and the Dicetel treatment group (P>0.05), The content of substanceP in organization homogenate of the ileocecal junction was significantly reduced inthe HSE treatment group than the Dicetel treatment group (88.29±5.51vs 135.11±34.66; P<0.05).CONCLUSION:HSE can reduce the number of mast cells in ileocecal junction and the contentof substance P in serum and organization homogenate of the ileocecal junction, tothe treatment effect on D-IBS.