The Influnce Of CO₂Gasless Laparoscopic Environment On The Proliferation Jiuman Ovarian Cancer Cell Strain SKOV₃

Objective: To investigate celoscope CO₂pneumoperitoneum's influence onovarian cancer cell strain SKOV₃growth and the possible molecular mechanisms andimpact of time limitation. To evaluation of celoscope CO₂pneumoperitoneum'ssecurity of the application in ovarian cancer.Method:(1)Establish an in vitro simulated CO₂pneumoperitoneum environment,exposing in CO₂environment with different pressures (8mmHg and12mmHg) for2hand4h respectively. Then separate from the pneumoperitoneum environment and putthe cells into the incubator of5%CO₂and continue to culture24h,48h,72h and14d.(2)Use the MTT Colorimetry to the OD value for the control group and aboveexperimental group Continuing to regular training24h,48h and72h(.3)Use the flatcloning for the experiment to examine the Cloning formation rate of the control groupand above experimental groups.(4)with western-bloting protein imprinting detection:â‘ the control group and above4experimental groups;â‘¡the experimental group of12mmHg CO₂environment cultivating4h, regular cultivate for24h Regular cultivateto24h,48h and72h and the control of Cycle CyclinD₁proteinand (cyclinD₁)andVascular Endothelial Growth(VEGF).Result:â‘´The OD value of the ovarian cancer cell line SKOV₃in8mmHg CO₂artificial gasless laparoscopic continuing role respectively2h,4h then Continuing toregular training24h and48h, respectively, were significantly higher than those inthe control groupï¹™P＜0.05﹚Continuing to regular training72h respectively, TheOD value of the experimental groups were compared with the control groups werenot statistically different(P＞0.05).⑵The OD value of the ovarian cancer cell lineSKOV₃in12mmHg CO₂artificial gasless laparoscopic continuing role respectively2h,4h then Continuing to regular training24h and48h, respectively, weresignificantly higher than those in the control group OD valueï¹™P＜0.05﹚Continuing to regular training72h respectively, The OD value of the experimentalgroups were not statistically different compared with conventional groups(P＞0.05).⑶The cloning formation rate of ovarian cancer cell strain SKOV₃in8mmHg and12 mmHg CO₂artificial gasless laparoscopic continuing role respectively2h,4h thencontinuing to regular training14d respectively, each cell of the experimental groupand the control group were not statistically differen(tP＞0.05).â‘·Ovarian cancer cellline SKOV₃in8mmHg and12mmHg CO₂artificial gasless laparoscopic continuingrole respectively2h,4h then continuing to regular training24h respectively, theprotein cyclinD₁and VEGF expression of SKOV₃cells were significantly higherthan those in the control group.The experimental group of12mmHg4h in CO₂gasless laparoscopic environment then continuing to regular training72h, the twoproteins was not statistically different compared with the control group(P＞0.05).Conclusion：⑴CO₂gasless laparoscopic environment can promote theproliferation of SKOV₃ovarian cancer cell and these changes can be reversible;⑵CO₂gasless laparoscopic environment can promote cyclinD₁and VEGF increaseprotein expression, The clinical effect is reversible in4h in CO₂gasless laparoscopicenvironment;⑶CO₂gasless laparoscopic environment can promote the proliferationof SKOV₃ovarian cancer cell and its mechanism may be through promoting cyclinD₁and VEGF protein expression increase, The changes can be reversible, witch showthat CO₂gasless laparoscopic surgery in the application of ovarian cancer is safe.