The Correlation Between Expression Of MMP-9and Beclin1in Colorectal Cancer And Its Clinical Significance

Objective: Colorectal cancer (CRC) is one of the most commonmalignant of digestive tract in China. In recent years, the studies found thatmatrix metalloproteinase-9(MMP-9) is one of the important gene in tumordevelopment, and plays an important role in tumor invasion and metastasis.Autophagy related proteins Beclin1is a key protein in the autophagyregulation, and is the marker of the tumor cell autophagy activity.In this study, MMP-9and Beclin1were used to evaluate the tumorinvasion and autophagy activity, to explore the relationship of the two factorsand the clinical significance in colorectal cancer development.Methods: The cancer tissues and matched normal tissues from30casesof patients with colorectal cancer were collected at the Fourth Hospital ofHebei Medical University in March2011-September2011. All specimenswere pathologically confirmed. The specimens were stored at-80℃. WesternBlot was used to detect the expression of MMP-9and Beclin1in colorectalcancer tissues and matched normal tissues. Using statistical softwareSPSS16.0analyzed the differences of MMP-9and Beclin1in cancer andnormal tissues, and their relationship with Dukes stage, location, and lymphnode metastasis. Spearman statistical method was used to detect thecorrelation of the two factors.Results:1The expression of MMP-9and Beclin1in colorectal cancer andmatched normal tissues.In our analysis of30cases, the expression of MMP-9(P<0.01) andBeclin1(P<0.05) in the cancer tissues has25cases and22cases, respectively,which was higher than that in the matched normal tissues in the sameindividual, The proportion is83.33%(25/30)and73.33%(22/30). The results suggest that the colorectal cancer cell invasion and autophagy activityincreased, compared with normal tissues.2The expression of MMP-9in Dukes C stage was significant higher thanDukes B stages and Dukes A stages(P<0.05),the expression of MMP-9incolorectal cancer Dukes A stage, Dukes B stage, Dukes C stage was higherthan that in the matched normal tissues, the proportion is40.0%(2/5),85.71%(12/14)and100%(11/11), respectively. The expression of Beclin1was no differences between Dukes C stage, Dukes B stages and Dukes Astages(P＞0.05).The expression of MMP-9in lymph node metastasis was significancehigher than non-lymph node metastasi(sP<0.05), the expression of MMP-9incolorectal cancer lymph node metastasis and non-lymph node metastasis has11cases and12cases was higher than that in the matched normal tissues, theproportion is100%(11/11) and73.68%(14/19). The expression of Beclin1have no differences between lymph node metastasis and without lymph nodemetastasis(P＞0.05).The expression of MMP-9have no differences between colon cancer andrectal cancer(P>0.05). The expression of Beclin1in rectal cancer was higherthan colon cancer(P<0.05). the expression of Beclin1in rectal cancer andcolon cancer has16cases and6cases was higher than that in the matchednormal tissues, the proportion is88.89%(16/18) and50.0%(6/12).3The expression of MMP-9and Beclin1in colorectal cancer tissuesparallel to elevated number is21cases, the proportion is70.0%(21/30). Therewas significant positive correlation between MMP-9and Beclin1in colorectalcancer (P<0.01).Conclusion:1The colorectal cancer cell invasion and autophagy activity increased.2The tumour invasion was positive correlation with the progress of thecolorectal cancer, and has no correlation with tumour location. The autophagyactivity of colon cancer cell was lower than rectal cancer, and has nocorrelation with Dukes stage and lymph node metastasis. 3The tumour invasion was significant positive correlation with thetumour autophagy activity (P<0.01).