Expression And Significance Of Stem Cells Marker CD133,Nestin In Brain Metastatic Tumor

Objective: To detect the expression of CD133,Nestin in brain metastatictumors, and to explore their role in the process of formation and the invasionof metastases,and further to identify the bionomics of tumor stem cells.Methods:1To collect the samples of brain metastatic tumors by surgical resection from52cases and samples of normal brain tissue10cases(normal brain tissues bysurgical resection from interior decompression in patients with craniocerebralinjury). All of the tissues were fixed in10%neutral formalin, embedded inpetrolin in generally and cut into slices serially. To retrieve correspondingpatients with primary tumor paraffin blocks of tumor specimens archived inpathology department, cut into slices serially.2Set of brain metastatic tumor and primary tumor for the study group andnormal brain tissue as the control group. And according to age, gender orprimary tumor site set the control group respectively. The detection ofCD133,Nestin was made by immunohistochemical Streptavidin-Persidase(SP) technique in brain metastatic tumor, primary tumor and normal braintissue. Each section at high magnification (×400) count10high power field,according to the percentage of positive cells assessed positive rating, tocalculated the percentage of positive cells.3Collect clinical pathology data of all the samples. Processing the acquireddata and analyzing the correlation between CD133,Nestin in brain metastatictumor with SPSS13.0statistic software. Apply chi-square for qualitative data;apply Spearman's rank correlation analysis for rank correlation test; set thesize of test is α=0.05; when P<0.05to have statistical significance.Results:1The positive rates of CD133,Nestin9expression in52cases of brain metastatic tumor were76.9%(40/52),80.8%(42/52)respectively. Thepositiverates of CD133,Nestin expression were70.00%(7/10),80.00%(8/10)in10cases of primary tumor archived in pathology. There was no expression ofthese two proteins in normal brain tissue.2The expression of CD133,Nestin in brain metastatic tumor and primarytumor were both significantly higher than normal brain tissue(P<0.05).Thedifference between brain metastatic tumor and primary tumor was nostatistical significance in expression of CD133,Nestin(P>0.05). Divided thepatients into different groups based on age, gender and primary tumor site, theexpression of CD133,Nestin in the different groups in brain metastatic tumorwere no statistical significance(P>0.05).3The correlation analysis of CD133,Nestin expression for all the brainmetastatic tumor samples showed that there was a significant positivecorrelation between CD133and Nestin.(γ=0.367,P=0.016)(γ=0.306,P=0.023).Conclusion: Tumor stem cells marker CD133, Nestin were overexpressed in brain metastatic tumor and primary tumor, and they were notexpressed in normal brain tissue. The over expression of CD133,Nestin inbrain metastatic tumor was not associated with primary tumor site, age andgender. There was a significant positive correlation between CD133andNestin, to provide condition for the formation of metastatic tumor. Theexpression of CD133,Nestin between metastatic tumor and primary tumorhave a significant positive correlation. The over expression of CD133,Nestinplayed an important biological function for the formation of brain metastatictumor, and the two proteins had a role of synergies regulatory. Proved theexistence of tumor stem cells in brain metastases.