| Objectives:To research the expression of the CCR7and D2-40in the thyroid carcinomas tissues; To explore the expression of the CCR7and D2-40in the thyroid carcinomas and the lymph node metastasis along with its characteristics of clinical pathology; To provide some theoretical bases for the clinical diagnosis and the treatment of TC and estimating the risk of lymphatic metastasis; to search a new target and to provide new ideas and methods which may be used to treat thyroid carcinomas.Methods:1) By means of application of the MAX Vision immunohistochemical staining technique, the expression of the CCR7was measured in the87cases thyroid carcinomas and8cases of thyroid tissues.2) Via application of the MAX Vision immunohistochemical staining technique, the expression of the lymphatic endothelial markers—2-40was measured in the87cases thyroid carcitarnomas and8cases of thyroid tissues,analysede and observed and located the lymphatic vessels in the light microscope.3) To proceed statistic analysis about the expression of the CCR-7and the D2-40in the thyroid carcinomas tissues and the related clinical pathological factors.Results:1,Pale brown Particles marked by CCR7were seen in the Cell plasma and cell membrane. CCR7was66.7%positive (86/104)in the87cases the thyroid carcinomas and CCR7was25%positive (2/8) in the8cases normal thyroid tissues, P=0.019(P<0.05).2,The CCR7positive rate of the PTC, MTC, FTC, UTC were counted up to75.0%,70.0%,73.0%,25.0%, P=0.012(P<0.05).3,The CCR7was81.6%positive (40/49)in the49cases lymph node metastasis positive patients and47.4%positive (18/38)in the38cases lymph node metastasis negative patients, P=0.000(P<0.05).4,The lymphatic vessels marked by D2-40were rarely seen in the intratumoral tissues of the thyroid carcinomas. The lymphatic vessels mainly distributed in the peritumoral tissues the thyroid carcinomas. The MLVD counts of the thyroid carcinomas and the normal tissues are5.72±2.89;2.60±1.14,The MLVD counts of the thyroid carcinomas obviously more than that of the normal thyroid tissues (P<0.05).5,The MLVD counts of the PTC, MTC, FTC, UTC are respectively7.55±2.29;6.60±1.75;4.27±1.10;1.00±0.34(P<0.05) It has a statistically significant difference.6,The MLVD counts of the49cases lymph node metastasis positive patients obviously more than that of the38cases lymph node metastasis negative patients (9.39±4.24>3.24±1.67,P<0.05).7,The expression ofthe MLVD marked by D2-40and the CCR7is positively correlated (coefficient of correlation, rs=0.645, P<0.000). The MLVD counts in the group of the CCR7-positive (9.37±2.25) is more than that the group of the CCR7-negtive (5.51±2.47).The difference was of statistical significance(P<0.05).8, The expression of the MLVD and the CCR7counts nothing to do with these factors:age,T-stage. They are connected with lymph node metastasis and Pathological type.Conclusion:1,The D2-40has specificity and sensitivity to lymphatic endothelial cells, and it can make a distinction between lymphatic vessels and vascular vessels, and can mark clearly tumor lymphatic vessels. The tumor lymphatic vessels mainly distributed in the peritumoral tissues of the thyroid carcinomas, which can apply to research about the tumor lymphatic vessels.2,The positive rate of CCR7and the MLVD marked by D2-40in the TC are more than the group of the normal thyroid tissues.3,In the thyroid carcinomas, It is different of the positive rate of CCR7and the MLVD marked by D2-40,PTC>MTC,FTC>UTC.4,The CCR7and the MLVD are positively relately with lymphatic metastasis, In the TC, The positive rate of CCR7and the MLVDof the lymph node-positive group is more than that of the lymph node-negative group.5,In the TC, The CCR7and the MLVD is also positively related, The MLVD of the CCR7-positive group is more than that of the CCR7-negative group.6,The CCR7and the MLVD have nothing to do with age, T-stages. They are connected with lymph node metastasis and Pathological type.In the thyroid carcinomas, In the lymph node metastasis positive-group,The expression of the CCR7is positively related to the MLVD marked by D2-40.The D2-40staining only presents in the lymphatic endothelial cells. Cancer cell express CCR7, The detection of CCR7along with the D2-40are expected to as inchoate and more effective signal to judgment lymph node metastases. It might provide strong theoretical bases for the treatment thyroid carcinomas and the lymphatic metastasis. |
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