Study On Peripheral Blood HLA-DR Antigen Expression And Lymphocyte Subsets Among Family Members Of Children With Recurrent Abdominal Pain

Objective:To investigate the prevalence of Helicobacter pylori(Hp) infection,the human leukocyte antigen HLA-DR expression and Lymphocyte subsets of the peripheral blood among family members of children with recurrent abdominal pain. To explore the association among HLA-DR expression, Lymphocyte subsets and Hp-associated recurrent abdominal pain.Methods:Hp immunophenotyping method was used to detect the Hp type of74family members and53normal controls. EDTA Venous blood specimens of74family members and37normal controls were obtained for lymphocytes and monocytes HLA-DR expression by flow cytometry. EDTA Venous blood specimens of all individuals were obtained for lymphocyte subsets analysis by flow cytometry. Individuals were divided into three groups:children with recurrent abdominal pain, adult members with recurrent abdominal pain and adults without recurrent abdominal pain. According to whether or not Hp infection, individuals were divided into the children with Hp-positive abdominal pain, adult members with Hp-positive abdominal pain, adults with Hp negative no abdominal pain.Results:1. The results of Hp immunophenotyping antibody test:The positive rate of Hp immunophenotyping antibody test in127individuals was64.6%.71were the Hp â… type,36with abdominal pain. The abdominal pain caused by the Hp I type accounted for50.7%.11were the Hp11type.5with abdominal pain. The abdominal pain caused by the Hp â…¡ type accounted for45.5%. The strain type of Hp has no effect on abdominal pain (x2=0.105, P=0.746)2. HLA-DR expression of peripheral blood:â‘ HLA-DR expression of peripheral blood lymphocytes has no difference (F=1.013, P=0.367) among children with recurrent abdominal pain, adult members with recurrent abdominal pain and adults without recurrent abdominal pain, but HLA-DR expression from monocytes was different (H=10.676, P=0.005) and was higher in children with recurrent abdominal pain than adults without recurrent abdominal pain.â‘¡HLA-DR expression of peripheral blood lymphocytes has no difference (F=1.200, P=0.307) among children with Hp-positive abdominal pain, adult members with Hp-positive abdominal pain, adults with Hp negative no abdominal pain, but HLA-DR expression from monocytes was also different (H=11.205, P=0.004) and was higher in children and adult members with Hp-positive abdominal pain than in adults with Hp negative no abdominal pain.3. The results of lymphocyte subsets on peripheral blood:â‘ The expressions of peripheral blood CD19+and CD4+were difference (F=8.583P=0.000) and (F=4.373P=0.015) among children with recurrent abdominal pain, adult with recurrent abdominal pain and adults without recurrent abdominal pain. Compared with adult with recurrent abdominal pain and adults without recurrent abdominal pain, CD19+was in increase in children with recurrent abdominal pain. CD4+was higher in adult with recurrent abdominal pain than in adults without recurrent abdominal pain. There was no significantly different about CD3+, CD8+,Th/Ts,and CD16+56in three groups.â‘¡children with Hp-positive abdominal pain, adult members with Hp-positive abdominal pain, adults with Hp negative no abdominal pain, CD19+was difference among three groups (F=10.374P=0.000). Compared with adult members with Hp-positive abdominal pain and adults with Hp negative no abdominal pain, CD19+was in increase in children with Hp-positive abdominal pain. There was no different about CD3+, CD8+, Th/Ts, CD4+,and CD16+56in three groups.Conclusion:1. The seropositive rate of Hp antibodies is high in the family members with recurrent abdominal pain. There is familial cluster phenomenon in family members suffering from recurrent abdominal pain.2. HLA-DR expression of peripheral blood monocytes is higher inchildren with recurrent abdominal pain than in adults without recurrent abdominal pain.3. HLA-DR expression of peripheral blood monocytes is also higher in children and adult members with Hp-positive abdominal pain than those adults with Hp negative no abdominal pain.4. Compared with adult with recurrent abdominal pain and adults without recurrent abdominal pain, CD19+was in increase in children with recurrent abdominal pain. CD4+is higher in adult with recurrent abdominal pain than adults without recurrent abdominal pain.5.Compared with family members with Hp-positive abdominal pain and adults with Hp negative no abdominal pain, CD19+was in increase in children with Hp-positive abdominal pain.