Expression And Significance Of IGF-â…¡ And BFGF In Adrenal Disease

Objective:To investigate the expressions of insulin-like growth factorⅡand basic fibroblast growth factor in adrenal disease by immunohistochemistry. to explore their course of disease in the adrenal role and clinical significance. Methods:1,To collect 75 conventional paraffin wax tissues of adrenal pheochromocytoma, corticomedullary hyperplasia, cortical adenoma, normal adrenal tissue from our hospital, from January 2003 to May 2010. and normal adrenal corned from corpse. Every group is 15 cases. Patient group with age range 21-56 years,22 males and 23 females. There were no clinical data show that concurrent other diseases.2,Detected by immunohistochemistry has been diagnosed with colorectal cancer pathology biopsy, with IGF-Ⅱantibody and bFGF antibodies that were 100 times and 300 times,100 times and 200 times diluted. IGF-Ⅱantibody and bFGF antibodies were 300 times and 200 times dilution, Because of dye clear and less background interference. And select a clear expression of the positive particles as positive control sections. Thick serial sections of 5μm, One for HE staining, the other with a 1:300 dilution of anti-IGF-Ⅱdo immunohistochemistry and 1:200 dilution of an anti-bFGF immunohistochemistry done. PBS solution instead of primary antibody as negative controls.3,After resting, sitting, mercury sphygmomanometer blood pressure 3 times, and the average value of 3 times.4,IGF-Ⅱand bFGF in the cytoplasm or nucleus within each group there were positive cells brown particles. In the section left, lower left, upper right, lower right, take a perspective, randomized, application "Image-Pro Plus 6.3" software to calculate the percentage of positive cells, and then averaged.5,SPSS13.0 statistical software was used for analysis. A one-way analysis of variance comparing the percentage area of positive cells in four groups, and then using SLD and SNK multiple comparison method. Each of IGF-Ⅱ, bFGF positive correlation between cell area using Peason product-moment correlation analysis. Using the same method to analyze the area of positive cells and the blood pressure link. When P <0.05 statistically significant difference.Results:The expression of IGF-Ⅱlevels and the expression level of bFGF expression in the four groups there was a significant difference (p<0.05) Descending order of expression is the human adrenal pheochromocytoma group, adrenal cortex and medulla hyperplasia, adrenal adenomas (Between groups:F=138.657,P=0.000和F=158.103,P=0.000). However, the group with normal adrenal glands to low expression or no expression (7.79±1.52 and 12.72±1.68, among groups p<0.05).Case group, IGF-Ⅱexpression and bFGF expression was positively correlated (p<0.05). Case group, IGF-Ⅱ-positive cell area positively correlated with blood pressure. bFGF-positive cell area is also positively related to blood pressure. (r=0.734and0.736,0.776and0.728 0.796and0.740)Conclusion:1,In the human adrenal cortex and medulla hyperplasia, cortical adenomas, especially pheochromocytoma, high expression of IGF-Ⅱand bFGF in the development of the disease may play an important role. At the same time there is a synergistic effect between the two.2,IGF-Ⅱand bFGF on the formation of those diseases play a role in hypertension.