Third Yellow Soup, Winter Protection Mechanisms Of Acute Renal Failure, Renal Tissue Of Rats

Objective:By the Treatment of oliguria of animal models of acute renal failure, it is the coction of Dong Di San Huang. It focused on studying experimental rat model of acute renal failure protective effect of kidney tissue and from serum creatinine, serum urea nitrogen,β2-microglobulin, organizational forms of kidney and so on to depth analysis; the treatment of acute renal failure may be the mechanism for the clinical application of the scientific experiments provided data for emergency medical treatment in severe reference.Method:1 Experimental groups:half of male and female rats of SD, the body is weighted among 300g±20%, were randomly divided into normal group, model control group, negative control group, the treatment group of chinese medicine, the treatment group of western medicine, the combination therapy group.2 Acute renal failure model's establishment and treatment way:In addition to the normal group, the remaining rats were cut water 24h, and the posterior limbs of intramuscular injection of 50% glycerol saline 8ml/kg. In acute renal failure animal model (2-3 hours):Chinese medicine treatment group was fed with the coction Of Dong Di San Huang; western medicine group were fed PF and energy mixture; in the combination therapy group were fed Dong Di San Huang soup in half an hour to give energy mixture and PF; negative control group was fed with 5% sugar, salt. Frequency of each group were administered 3 times a day. Under normal feeding in the natural environment; model group is not treated, and taking of blood and kidney after 48h.3 Drawn:Rats in each group using sodium pentobarbital anesthesia, the femoral artery blood, into the biochemical tube, and the removal of kidney, placed in neutral 4% paraformaldehyde solution to be fixed.4 Observation indicators and methods:The rats were placed in metabolic cages, Records the number of the death every day, after the animal molding completes. Observation of urine color, measure the volume of urine and stool, the use of automatic biochemical analyzer in the detection of serum biochemical. Are indicators of urea nitrogen and creatinine andβ2-microglobulin; production biopsy, HE stain, light microscopy of renal pathological change.Finally:1 Dong Di San Huang Tang groups compared with the negative control group mortality was significantly lower,the chinese medicine group survival rate was 75% and the negative control group survival rate was 25%, there were significant differences between the two groups. 2 The serum creatinine of Dong Di San Huang Tang groups,western medicine group,the combination therapy group are recovered to the normal range.3 HE staining showed that the pathological examination of Dong Di San Huang Tang groups is more smooth tubular, blocking less, but focal tubular epithelial cells were injured, some glomerular hyperemia; western medicine more complete renal tubular epithelial cells, but there are tubular partial obstruction, focal glomerular hyperemia; Chinese and western medicine combination group than the smooth tubular, Less destruction of epithelial cells, glomerular focal hyperemia; negative group's tubular is stenosis, severe glomerular congestion;Conclusion:Dong Di San Huang Decoction could significantly increase the survival rate of rats with acute renal failure, reduce serum creatinine and blood urea nitrogen, thereby improving renal function, and can affect the renal tissue of rats with acute renal failure morphological changes.