| Objective To investigate the effect and its mechanism of mitochondrial calcium uniporter on cerebral ischemia/reperfusion (I/R) and its mechanism.Methods The I/R models of rats were performed by thread embolism of middle cerebral artery for 2 h of reperfusion.40 rats were randomly divided into four groups: sham-operated group, ischemia group, ruthenium red group and spermine group. After 24 h of reperfusion, neurological score,and content of malondialdehyde(MDA), activities of lactate dehydrogenase (LDH) and superoxide dismutase (SOD) content in serum, the changes of positive cells in cortex, which contains Caspase-3 protein by immunohistochemistry were observed in the above groups respectively. All data were expressed as mean±SD. Statistical analysis was carried out by one-way ANOVA.Results The neurological score of the last three groups increased, serum MDA, LDH was significantly higher than the sham-operated group, and SOD was significantly lower. Immunohistochemistry showed that the expression of caspases-3 cells significantly increased compared with the sham-operated group. Compared with ischemia group and spermine group, ruthenium red, which was the inhibition of MCU, could significantly change the results to be better, while the opposite result of spermine group.Conclusion The inhibition of mitochondrial calcium uniporter can significantly protect the rat's brain by reducing the oxygen free radicals, maintaining mitochondrial function. |
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