The Different Habitats Sectional Reddish Brown And Off-white Soil Fuling Pharmacognosy

In Chinese pharmacopoeia (2010 edition) the Smilacis Glabrae Rhizoma and preparation of TCM clinical wide application. In recent years, with the deepening of the research of the traditional Chinese medicine, has many extraction, the chemical composition and the pharmacodynamic series research, thus for development has good curative effect, low toxicity of new drugs provided many new theory basis.In Chinese pharmacopoeia (2010 edition) Smilacis Glabrae Rhizoma section record of white to reddish brown, but in the process of collecting herbs found in the same species and different color in Smilacis Glabrae Rhizoma cross-section of the 20d have bigger difference, and this subject to section to red and white Smilacis Glabrae Rhizoma pharmacognosy and contain ingredients benchmark content of mangiferin bride falls to find section methodology of white to red brown Smilacis Glabrae Rhizoma pharmacognosy, and composition in aspects of difference.The design idea of the experimental study of section for:1. Smilacis Glabrae Rhizoma and Red Cross section on the Smilacis Glabrae Rhizoma white macroscopic and microscopic identification, intends to find out the difference between them, see whether there are specificity identification features; 2. If the differences of different sections, continues to Smilacis Glabrae Rhizoma characteristic on TLC identification of research, Smilacis Glabrae Rhizoma different section color respectively establi-shed the thin layer of herbs characteristic map; 3. Smilacis Glabrae Rhizoma section red and white of the indexes fall medicinal ingredients separately HPLC (bride content determination and the methodological research, and to many different origin Smilacis Glabrae Rhizoma medicinal materials for the content determination.Medicinal material, through cultivars identification in guizhou and throughout the country harvesting a sufficient number have fully representative sample of medicinal materials, research Smilacis Glabrae Rhizoma source and microscopical identification, find out Smilacis Glabrae Rhizoma section between white and Red Cross section medicinal identification features specificity, in an attempt to further chemical components and pharmacodynamics experiment aspects to confirm their differences.Establish thin layer, characteristic map using cosine method and the correlation coefficient method to calculate the similarity, two methods of calculating the similarity calculation income are greater than 0.95 so basic similarity is higher. Results show that the red and white cross section between chemical composition medicinal section is a large differ-ence between.Smilacis Glabrae Rhizoma by reveed-phase HPLC down the content of mangiferin bride, reference 2010 edition of China pharmacopoeia Smilacis Glabrae Rhizoma content determination of chromatographic conditions and the product preparation methods of optimization, the result in methodo-logy acetonitrile-0.1% glacial acetic acid solution as mobile phase, according to the Hypersil ODS2 chromatographic column, each sample chromatography separation, income is good, separation in fengfeng form best effect. Ultimately the content determination results show that section Smilacis Glabrae Rhizoma fell bride red (5.91%～2.08% in content range,3.89%, each batch norm is the content of different degrees of higher minimum 2010 edition pharmacopoeia 0.45%. Section white (bride medicinal fall in 2.42% content range, average 0.20 percent for animals, and four of them are batch crude content was lower than the minimum 2010 edition pharmacopoeia, so both there are distinct differences in the content.