Preparation And Properties Of A Chitosan/Tricalcium Phosphate Scaffold For Tissue Engineering

ObjectivePreparation of chitosan/tricalcium phosphate composite scaffolds, as the pulp of the feasibility of tissue engineering scaffolds.MethodTo prepare chitosan/tricalcium phosphate scaffold in freeze-dried method Chitosan powder was dissolved in 1% acetic acid solution and stirred into uniform foam. The pore size and vacancy volume of the wire frame were determined under light microscope, a cross-sectional morphology observed by scanning electronic microscopy (SEM). Composites were measured porosity, pore size distribution, compressive strength; test composite materials on cytotoxicity, combined with the cell culture to determine their biocompatibility.ResultIn this experiment, chitosan/tricalcium phosphate scaffold was fabricated by a method of freeze-drying, the scaffold's pore structure showed that its porosity rate was 85.78% on average, the maximum porosity rate is up to 90%, pore size is in 100μm-300μm, the composite scaffold material has good toughness, when the axial compression length was more than 5 mm, the material was still not be damaged., the results of cytotoxicity test were grade 0.Cell-culture with the scaffolds, showed that pulp cells closely attached to them and extend pseudopodiaConclusionIn this study, through the synthesis of chitosan/tricalcium phosphate composite materials, and determine its relative performance, a preliminary study of chitosan/tricalcium phosphate composite material with good biocompatibility, cell affinity and a certain mechanical properties to meet the basic requirements of biological materials.