| Objective:1.Through the paraffin serial section method, combining computer technique, we got the vestibular three-dimensional geometrical model which clearly displays the vestibular organs: semicircular ducts, utricle and sacculus etc. The model can deepen clinical doctors' accurate quantitative understanding of the macro and mesoscopic morphological structure of the vestibule,and it also lays the foundation for establishing animal experiment model under the abnormal conditions and applying to human experiments.2.Mastering paraffin serial section technology and computer reconstruction method, which can provide a tool for accurate measurement,evaluation of each vestibular structural parameters and the establishing of biomechanical numerical model .3.Give a numerical simulation in temperature field on the vestibular 3d model of Guinea pig and analyze the mechanical response in vestibular membrane film lost under the temperature stimulation. Then combined with clinical bithermal caloric test, the numerical simulation can help quantitative understanding the balance function of the vestibular system, and provide certain reference for the diagnosis of vestibular disease.Methods: Using fresh temporal bone, brought to fixation, decalcification, dehydration, transparent, leaching in the wax, paraffin embedding and position, serial section in slicing machine, dyeing, sealing piece etc, won a set of paraffin serial section with a 6μm tablet thickness, obtaining cross-sectional 2-d image digital information with the digital video. With the computer finite element technique, we established a vestibular 3d reconstruction, containing: membrane semicircular canals, utricle, sacculus and so on. Then give the model data measurement and numerical simulation in temperature field.Results:1.Got a full set of serial section of guinea pig internal ear organization and the enlarge image data.2.With paraffin serial section technique, combining with computer software, we established a 3d vestibular membranous model of guinea pig. The model can clearly show vestibular fine structure and spatial character and even obtained vestibular 3d data.3.The result of the numerical simulation of temperature field shows the complex mechanical behavior in the vestibular system. This paper exerts three temperature intervention conditions:△T=+1℃,△T=+7℃and△T=-1℃, then observe the response of semicircular canals crest :displacement, velocity and pressure changes. Temperature increasing or reducing, displacement are gradually increasing and speed are gradually decreasing. When the temperature rises, pressure are gradually increasing; the temperature reduce, pressure decreases gradually. The above indexes are finally tend to be a final stable date. The higher the temperature is, the stronger the mechanical response. The displacement response of the crista ampullaris crest of three semicircular canals was observed under temperature stimulation: the response of the horizontal semicircular is the biggest, the displacement response of two vertical semicircular canals is relatively smaller, and the posterior semicircular duct is the smallest. Pressure drop is visible on the colored atlases mainly lay on horizontal semicircular canals. Which reveals that temperature mainly influenced the horizontal semicircular canal. During the clinical caloric test, the VOG records to horizontal direction eye shake. That indirect reflects horizontal semicircular canal got the main stimulus in the caloric test; the numerical simulation results of temperature field in my paper are consistent with the clinical caloric test observations.Conclusion:1.With serial section technology acquisition 2-d image information and computer reconstruction, the reconstruction model has sliced detail, and can reflect the space characteristics, but also can undertake 3d data measurement. It is a kind of improving methods of temporal bone morphology research level, lays the foundation for future morphological teaching and future 3d modeling study of multiple organs, multiple systems and organizations.2. Through the numerical simulation of the vestibular temperature field, we can quantitative understand the mechanism of balance and offer the data reference for estimating the functional status of the whole vestibular system and the entire semicircular.3. It is feasible that the 3d model established with paraffin serial section method and computer technology is used for numerical simulation. |
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