Effects Of Propofol On The Apoptosis And Activation Of NF-κB In Polymorphonuclear Neutrophil In Acute Lung Injury Induced By LPS In Rats

Objective: To observe the effect of intravenous injection of Propofol on the apoptosis and activation of NF-κB in polymorphonuclear neutrophil expression in LPS-induced acute lung injury in rats.Methods: Sixty SD rats were randomly divided into five groups: group C: control group,group ALI: model (LPS 5mg/kg,iv) and propofol intervention group (P1: LPS 5mg/kg + propofol 5 mg·kg-1·h-1,P2: LPS 5mg/kg + propofol 10mg·kg-1·h-1,P3: LPS 5mg/kg + propofol 15mg·kg-1·h-1). Acute lung injury was induced by intravenous administration of LPS (5mg/kg) in rats. Then different dose of propofol was infused continuously through mini-pump for two hours immediately. All rats were killed by exsanguination. We elevate lung injury through HE stain,W/D. PMN were isolated from peripheral blood by density gradient method. The apoptosis rate of PMN was determined by flow cytometric analysis. Western blot was applied to evaluate the expression of NF-κB.Results: 1.Lung injury was more severe after intravenous administration of LPS, it appeared to be interstitial edema, alveolar septum thickness, and alveolar wall distortion. After continuous infusion of propofol for 2 hours, lung injury alleviated apparently. Compared with group C, lung injury was severer in group ALI IQA and W/D increased significantly, while PMN apoptosis rate was decreased(P<0.05). Compared with group ALI lung injury was alleviative in propofol intervention group, IQA and W/D decreased (P<0.05).2. Blood gas analysis results show that, compared with controls, ALI model group, natural results PaO2 and oxygenation index was significantly lower (P<0.05), hint ALI group model preparation success; ALI model group compared with propofol treatment groups PaO2 and oxygenation index results laminated index increased significantly, and in a dose-dependent manner.3. Compared with group C, lung injury was severer in group ALI PMN apoptosis rate was decreased(P<0.05). Compared with group ALI , lung injury was alleviative in propofol intervention group, PMN apoptosis rate increased, which exhibited a dose dependent manner (P<0.05).Western blotting results showed that PMN Phospho-p65 increased significantly in model group compared with control group (P<0.05), and decreased in propofol intervention groups compared with model group, which exhibited a dose dependent manner (P<0.05). There was no statistically difference of PMN total-p65 in five groups(P>0.05).Conclusion: Propofol can attenuate acute lung injury induced by LPS in rats, and inhibit the activation of PMN NF-κB p65 and promoting of PMN apoptosis. Attenuation of acute lung injury might be related to the inhibition of PMN Phospho-p65 and the promoting of PMN apoptosis.