The Effects Of Prostaglantiain E1 To The Phenotype And Functional Activity Of Macrophage Which In Mouse Uterus

Objective: Through the observation of the influence of neurotransmitter of Prostaglandin E₁ to the phontype and function activity of macrophage in the uterus of normal mice, to explore its mechanism of immune suppression and the relationship between maternal-fetal immune tolerance.Methods:Non-pregnant normal Kunming mice were randomly divided into control group (A group) and three experimental groups E-SG, E-SZ, E-SD. Experimental group were intravenous 1303ng/kg/d, 2606ng/kg/d, 3909ng/kg /d of prostaglandin E(10.2ml),with a sterile saline(0.2ml) control group, continuous injection of 5 days. After the last injection, respectively 1h, 3h, 6h, 12h, 36h to take the uterus. Frozen sections and paraffin sections made and used non-specific esterase staining and immunohistochemical method to detec the phenotype and functional activity of uterine macrophages.Results:(1)Comparing with control group mice, After the injection of PGE₁for 1h, the expression of uterus macrophage CD14⁺ at the endometrium, myometrium and perimrtrium of every experimental group showed an apparent down-regulation (P<0.01). After the injection of PGE₁ for 3h, the expression of uterus macrophage CD14⁺ at the endometrium and perimrtrium of every experimental group showed an apparent down-regulation (P<0.01),the expression of macrophage CD14⁺ at the e perimrtrium of every experimental group showed an apparent increase. (P<0.01). After the injection of PGE₁for 6h, the expression of uterus macrophage CD14⁺ at the endometrium of every experimental group showed an apparent down-regulation (P<0.01).and the expression of uterus macrophage CD14⁺ at the perimrtrium of every experimental group showed an down-regulation (P<0.05).(2)There is no macrophage CD204⁺ found in control group and low concentration group. They only been found in medium experimental group after the injection of PGE₁ for 12 hour and 36 hour. They also been found in high experimental group after the injection of PGE₁ for 6 hour, 12 hour and 36 hour. CD14⁺ and CD204⁺ had a similar change tendency. The high experimental group after the injection PGE₁ for 36 hour had significant increase. (P<0.01).(3)Comparing with control group mice, After the injection of PGE₁ for 1h, the expression of uterus macrophageα-NAE+ at the endometrium of every experimental group showed an significant chage,Macrophageα-NAE+ at the perimrtrium had significant increase . After the injection of PGE₁ for 12h, the expression of uterus macrophageα-NAE⁺ at the endometrium of every experimental group showed an significant increase,Macrophageα-NAE⁺ at the myometrium had significant decrease .After the injection for 36h, the expression of uterus macrophageα-NAE+ at the endometrium, muscularis and outer membrane of high experimental group showed an apparent increase. Conclusion:(1)PGE₁ can inhibit the migration and activation of macrophage ,promote the migrate from the endometrium to the perimrtrium,and this effect within a certain range was positively correlated with the dose and half-life related. As time goes by, PGE₁ can promote he migration and activation of macrophage.(2)A certain concentration of PGE₁ can promote the expression of uterine macrophage receptor CD14⁺ and CD204⁺ .(3)PGE₁ regulate the activity and function of macrophage by the way of adjusting their amount and acceptors of CD14⁺ and CD204⁺ to take part in the occurrence and maintenance of uterine immune tolerance.