Establishment Of Subcutaneous Transplanted Tumor Model Of Skin Adenocarcinoma In Balb/c Mice

Objective:To establish a subcutaneous transplanted tumor model of skin adenocarcinoma in Balb/c mice and its tumor biological characteriztions.Methods:Tumor-bearing mice were killed by decapitation and tumors were collected under sterile conditions. Cell suspension was prepared from the tumor, and injected subcutaneously with concentration of 1x10'cells/ml into right lower limb near abdomen, (0.2ml each mouse). The tumor growth rates, the spontaneous metastasis, and the survival time of the tumor-bearing mice were determined. Tumor histology and immunochemistry were also examined for tumor identification.Results:The successful rate of the transplantation tumor model was 100% and the nature extinctive rate was 0%.There was no the spontaneous metastasis. The natural survival time in the transplantation tumor mode was 40-45 days.Conclusion:The subcutaneous transplanted tumor model of skin adenocarcinoma in Balb/c mice is an ideal model, just because of its successful rate with no nature extinction. Objective:To study the Anti-tumor activity of JS-K in vivo and to explore its possible mechanismMethods:A mouse experimental model of adenocarcinoma xenograft was developed and randomly divided into negative control group, JS-K group and the cisplatin group. The group were given intraperitoneal injection (ip) drug administration 20 days after inoculation of tumor cells for 24h.The last dose was given in the 21st day of tumor inoculation, Mice were killed by decapitation. Tumor was stripped to calculate the weight inhibitory rate.Caspase-3, Bax and Bcl-2 gene expression was detected by reverse transcriptase PCR, and Caspase-3,Bax, proteins expression were determined by Western blot.Results:Compared with the control group, the tumor weights in JS-K group and cisplatin groupwere significantly lower (P<0.05) In JS-K group and cisplatin group, the inhibition rates were 64.8% and 49.5%, respectively. Routine pathological examination showed that compared with the control group, treatment group had a wider range of apoptosis and necrotic area。Caspase-3, bax gene expression increased significantly in JS-K treated group as detected by real-time RT-PCR. The expression of caspase-3 increased by nearly three times, and the same as the results by western blotting method showed.Conclusion:JS-K inhibited the growth of adenocarcinoma xenograft, The inhibitory mechanism may be related.to up-regulate cell apoptosis and caspase-3, bax expression.