| Objective:To study the dynamic expression and location of HO-1 in rat pulpitis at different time .To evaluate the role of HO-1 with the development of dental pulpitis.Methods:Twenty SD rats were chosen and given into five groups. One group was normal control group, other four groups were experimental groups. Four groups rats accepted pulp exposure operation in the right maxillary first molar and right maxillary second molar. And they were sacrificed after exposure 1st,3rd,6th,9th day. The teeth of each animal were fixed, decalcified and section serially. The section was stained with HE and pathological changes were evaluated under a light microscope. The expression of HO-1 was detected in normal and injured pulp immunohistochemically.Results:1. Inflammation of pulp tissue was gradually serious after pulp exposure from 1 day to 9 day. Vascular hyperemia,infiltration of leukocyte, necrosis of odontoblast and reparative dentin were observed with development of pulpitis which is gradually necrotic from coronal pulp to radicular pulp.2. HO-1 was weak positive in the odontoblastic and fibroblast of normal pulp. It was positive in the odontoblasti and fibroblast after pulp exposure 1 day. The rest was negative. Conclusions:1. Experimental pulpitis model is established successfully because of histological change of experimental pulpitis after pulp exposure which accorded with nature development of pulpitis, It provides the base for further research of pulpitis.2. The weak expression of HO-1 in odontoblast and fibroblast in normal dental pulp shows that it takes part in dentin production and dental pulp cell metabolism of rat.3. The strong expression of HO-1 induced in 1 day group shows that it participates in anti-inflammatory action in the early stage of pulpitis and protects the pulp tissue. |
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