| Objective A number of articles have reported that mesenteric lymph blockage can decrease gut-induced lung injury in many animal models. However, few researchers focus on the process of inflammation resolution after mesenteric lymph blockage. The present study was designed to investigate the role of mesenteric lymphatic duct drainage in the inflammation resolution process of gut-induced lung injury in rats.Methods Male Sprague-Dawley rats were randomly assigned to intestinal ischemia-reperfusion (I/R) group, mesenteric lymphatic duct drainage (MLDD) before I/R group and sham-operated group. Rats were killed at designated time point to obtain BALF (bronchoalveolar lavage) and lung tissue samples. We documented inflammatory cells in BALF and cytokines in lung,and we introduced a series of resolution indices as main criterion to judge the speed of inflammation resolution.Results In 6 days experimental process, compared to I/R group, MLDD attenuated PMNs and reducedψmax (p<0.05), but did not bring forward T50 and shorten Ri (P = 0.152). At 6h and 1d, MLDD reduced PMNs, TNF-αand increase IL-10 (P < 0.05), whereas did not change their content at subsequent time point.Conclusion Mesenteric lymph duct drainage has ability of anti-inflammation and abrogates acute lung injury (ALI) after mesenteric I/R,but this treatment has not capacity to activate and accelerate inflammatory resolution in this model. |
PDF Full Text Request