Effects Of LBP On Contents Of Serum Cytokines In Female Osteoporosis Model Rats

Objective To study the effects of lycium barbarum polysaccharides (LBP) on contents of serum IL-6,TNF-α,NOS and TGF-β1 in female osteoporosis model rats,explore LBP curative effect, the onset time and possible mechanism. Methods 50 female SD rats with aged 3 months were randomly divided into 5 groups(10 rats in each group):SHAM group in which rats received sham surgery, OVX group in which ovariectomized rats didn't give any medicine after the removal of ovaries , E2 group in which ovariectomized rats administrated estrogen, L group in which ovariectomized rats administrated low doses of LBP and H group in which ovariectomized rats administrated high doses of LBP. All rats were bred two months after operation and then each groups followed by different medicines. The contents of serum IL-6 and TNF-αwere detected before treatment(T0), after 45 days (T1) of treatment in every groups . The contents of serum IL-6 ,TNF-α,NOS and TGF-β1 were detected after 90 days (T2)of treatment in every groups. Results At T0, the levels of IL-6 and TNF-αin SHAM group rats were significantly lower than other groups .At T1, the levels of serum IL-6 in H group and L group were lower than these of OVX group and higher than these of SHAM group and E2 group (P<0.05). At T2, the level of IL-6 in L group and H group were lower than OVX group(P<0.05),and have no significant change compared with SHAM group and E2 group(P>0.05), the level of IL-6 in H group lower than these in L group at T2 and H group at T1(P<0.05). Except E2 group, the level of serum TNF-αat T1 and T2 times had no significant change in other groups(P>0.05).At T2, the level of NOS and TGF-β1 in L group and H group were higher than OVX group(P<0.05),and have no significant change compared with SHAM group and E2 group(P>0.05). Conclusion 1 After 45 days of gavage intervention, high and low doses of LBP could reduce the serum IL-6 levels, And at 90 days, 40mg/kg ? d dose of LBP's effect is the best. 2 LBP may not through reducing the levels of serum TNF-αimprove bone metabolism disorder in osteoporosis rats. 3 Both high and low doses of LBP could increased serum levels of NOS and TGF-β1 to near normal, thus playing a role in regulating bone metabolism.