Efficacy Of Ciprofloxacin -poly(D, L-lactide-co-glycolide) Microspheres For The Adjuvant Treatment Of The Sd Rats Gustilo II Type Open Femur Fracture

1. Objective: The aim of this study was to prepare Poly(D,L-lactide-co-glycolide) (PLGA) biodegradable microspheres loaded with Ciprofloxacin(CIP-PLGAMS) by the double emulsion solvent evaporation method and to evaluate its efficiency for the adjuvant treatment of SD rats Gustilo II type femur open fracture.2. Methods: The microsphere size, morphological characteristics, production yield, drug content, encapsulation efficiency, in vitro release had been detected. The model of SD rats Gustilo II type femur open fracture infected with Staphylococcus aureus (ATCC25923) were created in 36 rats which were divided into group A (control group) and group B (experimental group). Sterilized CIP-PLGA microspheres and PLGA microspheres were implanted in the femur fracture site of rats in group A (control group) and group B respectively after debridement. After 4 weeks of treatment, the rats were analyzed with X ray and microbiological assay.3. Results: PLGA microspheres between the size ranges of 30-100μm were obtained. Production yields, drug content and encapsulation efficiencies were found to be higher than 70%, 2.80% and 13.75% respectively. In vitro drug release studies shown that these microspheres had significant burst release and their 28 days drug cumulative release rates were higher than 70%. Based on the in vivo data, cure rate of the rats implanted with CIP-PLGA microspheres and empty microspheres were 94.44% and 61.11% respectively (p < 0.05).4. Conclusions: CIP-PLGA microspheres were found to be effective for the treatment of Gustilo II type open fracture in SD rat experimental model. Therefore, these microspheres may be potentially useful in clinical medicine.