Effects Of Asiatic Acid On The Expression Of MRNA Of Smad7 And PPARγ In HSC-T6 In Vitro

Objective: To investigate the effects of Asiatic acid on the expression of mRNA of smad7 and PPARγin HSC-T6 in vitro and to explore the molecular mechanism of the anti-liver fibrosis activity of the Asiatic acid.Methods: The influence of asiatic acid on HSC proliferation was detected by MTT assay and optimum concentration was determined. The cultured HSC were divided into control group and Asiatic acid treatment group. The influence of asiatic acid on the expression of mRNA of smad7 and PPARγin HSC-T6 in vitro was detected by semi- quantitative RT-PCR.Results:The asiatic acid significantly inhibits the proliferation of HSC in a dose dependent manner. Compared with the control group, the expression of mRNA of smad7 and PPARγof HSC-T6 in vitro in the Asiatic acid treatment group significantly increased (samd7/GAPDHmRNA:0.267±0.037vs0.358±0.035;PPARγ/GAPDHmRNA:0.157±0.054 vs 0.570±0.066). There was significant difference between the two groups P<0.05.Conclusion :Asiatic acid inhibits the collagen synthesis and secretion in HSC and improves the pathology of liver fibrosis possibly by up-regulating the expression of mRNA of smad7 and PPARγ.