Protein And Genomie Study On MMP-9 And VEGF Of Adamantinomatous Craniopharyngioma

Objective: The aim is to study the proteine and mRNA expression of MMP-9 and VEGF in Adamantinomatous Craniopharyngioma, and the correlation between the expression of MMP-9, VEGF and the invasion and recurrence of tumors.Methods: The proteine and mRNA expression were tested by immunohistochemistry (IHC) and real-timePCR in 40 cases of adamantimous craniopharyngioma, including 24 cases of primary group and 16 cases of secondary group.Results: IHC showed: The total expression rates of MMP-9 in secondary group were significantly higher than primary group (93.7% vs 41.7% , P < 0. 05) ,and the total expression rates of VEGF in secondary group were significantly higher than the primary group (87.5% vs 45.8% , P < 0. 05). There were mutually positive correlations between MMP-9 and VEGF expressions in secondary adamantinomatous craniopharyngioma. and were no significant correlation in primary adamantinomatous craniopharyngioma,real-timePCR showed: The relative expression amount of MMP-9mRNA is obviously higher in the recurrence adamantinomatous craniopharyngiomas group(7.26±0.37) than in the primary group(3.75±0.50,P<0.01); and the relative expression amount of VEGF mRNA is obviously higher in secondary group were significantly higher than the primary group (7.26±0.37vs 2.90±0.54 P<0.01)) than in the primary group(3.75±0.50,P<0.01).Conclusion: MMP-9 and VEGF may be play an important role in the invasive growth and recurrence of adamantimous craniopharyngioma, and positive-feedback regulate each other. MMP-9 and VEGF maybe serve as important biomarkers to evaluate the inclination of invasion and recurrence in adamantimous craniopharyngioma...