Study On Correlation Between PTEN And PI3K/Akt Pathway In Endometrial Carcinoma

Objective:To investigate the status of the expression of PTEN, and the PI3K/Akt signaling pathway activation in endometrium tissues, combine with the expression of p53 and proliferation activity of Ki-67, and to explore the relationship of between PTEN and PI3K/Akt pathway with the mechanism of endometrial cancer.Methods:(1)The objects of this experiment, there were 54 cases of endometrial adenocarcinoma (EA),13 cases of uterine serous carcinoma (USC) and 12 cases of normal endometrium (NE); (2) The expression of PTEN,PI3Kp110α,p-AktSer473,p53 and ki-67 by the mmunohistochemistry method(S-P method), and analysis the correlation of the expression of proteins with the EA group and the USC group; (3) and analysis the relationship between the expression of proteins and muscle layer invasion depth.Results:(1)The loss expression rate of PTEN in EA group, USC group and NE group, respectively, were 79.63%,61.54% and 16.67%, the loss rate of PTEN were higher in patients with EA or USC than in NE group, respectively, the difference were statistically significant (χ2=13.711, P=0.000; P=0.041). With the increased depth of myometrial invasion, PTEN expression decreased, the positive correlation between PTEN expression with myometrial invasion (χ2=6.997, P=0.030). (2) The strong positive expression rate of PI3Kp110αin EA group, USC group and NE group, respectively, were 48.15%,46.15% and 0%, the strong positive rate of PI3Kp110αwere higher in patients with EA or USC than in NE group, respectively, the difference were statistically significant(χ2=7.623, P=0.006; P=0.015). (3) The positive expression rate of p-AktSer473 in EA group, USC group and NE group, respectively, were 88.89%,69.23% and 58.33%, the strong positive expression rate of p-AktSer473 in EA group, USC group and NE group, respectively, were37.04%,30.77% and 0%, the positive rate and the strong positive rate of p-AktSer473 were higher in EA group than in NE group, respectively, the difference were statistically significant(χ2=4.583, P=0.032;χ2= 4.744, P=0.029).(4) In EA tissue the strong expression of PTEN and PI3Kp110αproteins showed negative correlation (rs=-0.273, P=0.046); the positive expression of PI3Kp110αand p-AktSer473 proteins showed positive correlation (rs=0.836, P=0.000). (5) The positive expression rate of p53 in EA group, USC group and NE group, respectively, were 27.78%,100% and 0%, the positive rate of p53 were higher in patients with USC than in EA group and NE group, the difference was statistically significant(χ2=18.303, P=0.000; P=0.000). The strong positive expression rate of p53 in EA group, USC group and NE group, respectively, were 3.70%,84.62% and 0%, the strong positive rate of p53 were higher in patients with USC than in EA group and NE group, the difference was statistically significant(χ2=38.842, P=0.000; P= 0.000).(6). Ki-67 proliferation index higher in USC group than EA group and NE group, respectively, the difference were statistically significant between two groups (P=0.000).Conclusions:(1) The loss expression of PTEN may be associated with the pathogenesis of EA and USC, the positive correlation between the expression of PTEN protein and the depth of myometrial invasion, the loss expression of PTEN may be associated with the mechanism of invasion in EC. (2) The high expression of P13Kp110αand p-AktSer473 proteins may be associated with the pathogenesis of EA and USC, but no correlation between the expression of those proteins and depth of myometrial invasion. (3) The high expression of P13Kp110αin the EA group, may be related to decreased the negative regulation of PTEN, and the positive regulation of the expression of p-AktSer473. (4) The strong positive expression of p53, the high expression of Ki-67,have reference value of the differential diagnosis between poorly differentiated EA and USC. The strong expression of p53 and the high expression of Ki-67 may be more meaningful the moleculartargets in differential diagnosis of USC.