Variation Law Research Of Toosendanin Content In Different Melia Azedarach Linn. Species

The ultrasonic wave extraction and the highly effective liquid chromatography were used to determine the content of toosendanin in M. azedarach L. In research annual period change of the content of toosendanin from different spot, different time in M. azedarach and selects the high toosendanin content from family and clone and single. The main conclusions are as follows:(1) Fifiteen trees were randomly selected from Dafeng forest farm, in Dafeng city, Jiangsu province. They were used to determine the content level of toosendanin in bark, velamen, fruit, flower pedicel, petiole and leaf. The results indicated that all the parts of M. azedarach contain toosendanin and the content of toosendanin in bark and velamen is the highest;(2) The study that annual period change of the content of toosendanin in leaf, fruit, bark and velamen revealed that the content of toosendanin in leaf is the lowest level in October, the highest level in June, global analysis, the content of toosendanin in leaf is in late spring and early summer highest; the content of toosendanin in fruit is the lowest level in October, the highest level in July, global analysis, the content of toosendanin in fruit is in the summer highest; the content of toosendanin in bark is the lowest level In October, the highest level in December, the higher level in June, global analysis, the content of toosendanin in bark is in the summer highest;There are two peaks maximum content of toosendanin in bark, one is in late spring and early summer and the other is in late autumn and early winter. the content of toosendanin in velamen is the lowest level in March, the highest level in August, overall, the content of toosendanin in velamen is in from April to October highest.(3) Ten out of 32 M. azedarach families were selected; these are binhai080102,weifang080105,suqian071204,xuyi07112,ganyu071101,binhai080101,dafeng071101,lishui080103,weifang08012,dafeng080101; In 150 M. azedarach single trees, the four fine single trees of the highest toosendanin content in bark and leaf are choosed; these are ganyu071101-7, weifang080106-7, xuyi071102-6, xuyi071102-11, three fine single trees of the highest toosendanin content in bark are choosed, these are weifang080105-1, xuyi071102-2, weifang080105-11, ten fine single trees of the highest toosendanin content in leaf are chosen, These are ganyu071101-1, lishui080103-11, suqian071204-11, weifang080105-8, suqina071204-5, binhai071101-1, lishui080103-9, lishui080103-12, suqian071204-3, weifang080105-3, jiangning071206-5. In 21 M. azedarach clones, the clones of the highest content of toosendanin are 92 and 30; the clones of the highest content of toosendanin in fruit are 36 and 124; the clones of the highest content of toosendanin in leaf are 84,105,153,30,611,15 and 80.