Effects And Mechanism Of Polysaccharide Of Quanzhenyiqi Decoction On SIgA In Intestine Of Immune Suppression Mice

Objective1. To preliminarily study the effects of polysacchafide of Quanzhenyiqi(QZYQ) Decoction on the function of intestinal mucosal humoral immune.2.To study the immune pharmacological mechanism of QZYQPS, lacate the effection target or target organ and study the molecular mechanism.Method24 Health SPF KM mice,the female share the same number with the male,6⁸ weeks age,18²2g weight,were randomly divided into three groups: normal control group,model group,QZYQ polysaccharide(QZYQPS) group,N=8.QZYQPS group were administered intragastrically QZYQPS with 1g.kg^-1.d^-1 everyday,a total of 8d, the former two groups were given NS the same volume as the PS,at the seventh day, the later two groups were intraperitoneal injected CY with 100mg.kg^-1, normal control group were injected with saline the same volume as CY, 24hs after injection, all animals were killed by cervical dislocation, each group were detected the intestinal mucosal sIgA, Peyer, s patches, TLR4 receptors through the ELISA,FCM and IHC.The data were analyzed by SPSS16.0 statistical analysis software and the statistical method is One-Way ANOVA.Result1.Compared to the normal control group and QZYQPS group,the concentration of sIgA and the ratio of CD19+ positive lymphocytes were significantly decreased in the model group(P<0.01);the normal group and QZYQPS group had no statistics difference of the concentration of sIgA and the ratio of CD19+ positive lymphocytes,P>0.05.2. Compared to the normal control group and QZYQPS group,the expression quantity of TLR4 was significantly decreased in model group,P<0.01;the normal group and QZYQPS group had no statistics difference of the expression quantity of TLR4 receptors,P>0.05.3.The expression of sIgA is correlated with TLR4 in QZYQPS group,R=0.942,P<0.01。Conclusion1.Intraperitoneal injection of CY can copy animal model of intestinal immune suppression.2.QZYQPS can increase the inhibition of humoral immune function, has the effection of regulation of intestinal mucosal immunity.3.The immunomodulatory effects of QZYQPS may through the way of regulating intestinal mucosal TLR4 receptors.