Targeting Fusion Protein With Active Caspase6 Suppress The Growth Of HER2 Overexpressing Human Glioblastoma Cells

Glioma is the most common tumor of the brain. Though the aggressive surgery or radiotherapy, patient prognosis still poor. HER2 is over expressing in several human tumors. When it is active, HER2 promote the ability of tumor cells in metastasis and invasion. HER2 is negative in the human central nervous system. The over-expressing of HER2 is associated with malignant phenotypes of human gliomas. HER2 can be a target for targeted therapy of gliomas. We investigated the expression of HER2 in glioma cell lines A172, U251, U87 and SHG-44. We constructed a novel immune caspase6 which were fused with e23sFv, Fdt and active caspase6. The novel immune caspase6 can bind to and be internalized into HER2 overexpressed tumor cells, then cleaved by furin and translocated into the cytoplasm directly. Our investigation provides a novel strategy for the treatment of HER2 overexpressed gliomas.