Determination Of Nucleic Acids, Proteins And Bisphenol A By Resonance Light Scattering Technique

Nucleic acids and proteins are important life substances, their quantitative determination plays an important role in biology, chemistry, clinical and medical diagnosis fields. Establishing ultra-highly sensitive and selective methods for environmental hormone analysis and monitoring has practical significance. On the basis of the investigation of large numbers of references, this paper adopted thifensulfuron methyl-simazine, 2, 4-D-CTMAB as DNA probs, fosinopril sodium as bovine serum albumin (BSA) probs andβ-cyclodextrin (β-CD) as bisphenol A (BPA) probs, discussed the reaction mechanism by multi-spectroscopic methods and electron microscopic technology and proposed two new assays for fsDNA determination, one new assay for BSA determination and BPA determination which were simple, time-saving and with good selectivity and sensitivity.We used thifensulfuron methyl-simazine as the fish sperm deoxyribonucleic acids (fsDNA) resonance light scattering (RLS) probe, studied the properties of RLS spectra, fluorescence spectra, UV absorption spectra and Atomic force microscope (AFM) imagings, discussed the interaction mechanism and proposed a new assay for fsDNA determination by enhanced RLS signals. Under the optimum conditions, the enhanced RLS intensity was proportional to the concentration of fsDNA in the range of 0.025mg/L~4mg/L, the related coefficient is 0.9990, and the limit of detection was 3.23ng/mL.We used 2, 4-D-CTMAB as the fsDNA RLS probe, researched the interaction among them, discussed the reaction mechanism by RLS spectra, fluorescence spectra, UV absorption spectra and AFM technology and proposed a new assay for fsDNA determination by enhanced RLS signals. In pH11.0 BR buffer, the enhanced RLS intensity which located at 345nm was proportional to the concentration of fsDNA in the range of 0.2mg/L~1.6mg/L.This method was applied to determination of synthetic samples analysis with satisfactory results.We established an assay for trace determination of BSA based on the enhanced RLS technology of fosinopril sodium. By using RLS spectra, fluorescence spectra, UV absorption spectra and AFM technology, we discussed the reaction mechanism and researched the interaction between fosinopril sodium and BSA. In pH11.0 BR buffer, under the optimum conditions, the enhanced RLS intensity was proportional to the concentration of BSA in the range of 0.05mg/L~5mg/L. This method was successfully applied in the BSA determination.By utilizingβ-CD inclusion complexes, we determined BPA in landfill leachate in the use of RLS technology. In pH3.26 BR buffer,β-CD-BPA inclusion complexes induced the great enhance of RLS signals, which was proportional to the concentration of BPA in the range of 0.01mg/L~0.1mg/L and 0.1mg/L~10mg/L respectively. This assay has very good specificity to BPA.Based on the measurements of RLS spectra, fluorescence spectra, UV absorption spectra, AFM imagings and influences of experimental conditions, we discussed the combined effects of thifensulfuron methyl, simazine and 2, 4-D, CTMAB to fsDNA respectively and studied their biological toxicosis mechanism.