Pcr-ldr-nucleic Acid Test Strip Assay In 315 Mycobacterium Tuberculosis Katg Gene Codon Mutation Detection

Objective:This study aims to meliorate and optimize a novel molecular diagnositic method(PLNA), prove the feasibility of this method for detecting the site specific mutations of katG gene condon 315 of Mycobacterium tuberculosis , and apply this method to the distruction of clinical drug use .Methods:Primers and probes were designed and synthesized for the detection of mutations in katG gene codon315 of Mycobacterium tuberculosis , performed the LDR reaction , and then detected use the nucleic acid detection strip , the results were compared with PCR-RFLP and direct sequencing .Results:The wildtype and mutants were successfully diagnosed , a high consistency was found compared with PCR-RFLP and direct sequencing ,the concordance rate was 97.5% and 100% respectively .Conclusion:This study provided a viable low-cost quick molecular diagnosis method for the clinical detection of mutations at katG gene codon 315 of Mycobacterium tuberculosis.