Effects Of Curcumin On Proliferation, Invasion And VEGF-C Gene Expression In Breast Cancer MCF-7 Cells

Objective:To evaluate the effects of curcumin on proliferation, invasion and VEGF-C gene expression in Human breast cancer cell line MCF-7.To explore the molecular mechanism of the inhibitory effect of breast cancer metastasis by curcumin.Methods:1.Proliferation of MCF-7 cell inhibited by curcumin was assessed by MTT. IC50 value was calculated by drawing the growth curve.2.Invasion of MCF-7 cell inhibited by curcumin was measured by Transwell technique.3.Expression levels of VEGF-C mRNA in MCF-7 cell was determined by reverse transcription polymerase chain reaction(RT-PCR).4.Expression levels of VEGF-C protein in MCF-7 cell was determined by Western blot.Results:1.The Curcumin markedly inhibited configurition and proliferation capacity of MCF-7 cell (P<0.001). The half maximal inhibitory concentration (IC50) was 37μmol/L2.The number of invasive MCF-7 cells were significantly lower in curcumin group than control group (P<0.001).It was also lower in 40μmol/L group than 20μmol/L group(P<0.001).3.The Curcumin effectively downregulated VEGF-C mRNA expression in vitro.The relative expression of VEGF-C mRNA was lower in 40μmol/L group than 20μmol/L group (P<0.05).4.The Curcumin effectively downregulated VEGF-C protein expression in vitro.The relative expression of VEGF-C protein was lower in 40μmol/L group than 20μmol/L group(P<0.05).Conclusion:1.The proliferation and invasion ability of MCF-7 cell is effectively inhibited by curcumin.2.The Curcumin effectively downregulated VEGF-C mRNA and protein expression in vitro respectively.3.It is possible to inhibit the invasion of MCF-7 cell by down-regulation of VEGF-C gene expression.