Expression Of MicroRNA-486 In Patients With Acute Leukemia

BackgroundLeukemia is the most common hematological malignancy with rapid progression and poor prognosis. The incidence rate of leukemia in China is 4-7/10 millions. MicroRNAs (miRNAs) are an abundant class of 19-25 nt nucleotide small noncoding RNA molecules, which are widely found in eukaryotes. They can regulate gene expression through either inhibiting the translation or promoting the degradation of target mRNA. MicroRNAs have been implicated in control of proliferation, differentiation and apoptosis. Mounting evidence indicates that their expression might be associated with tumorigenesis, progression and resistance. MiR-486 has been found underexpressed in a variety of malignancies such as lung cancer, colon cancer, laryngeal cancer and so on. The relationship between miR-486 and AL is still unknown.ObjectivesIn the present study, we detect the expression of miR-486 in peripheral blood in patients with acute leukemia and health people, so we can investigate its roles in the pathogenesis and resistance of AL. The analysis may find that miR-486 as new cancer biomarker that could be useful for early diagnosis and therapeutics in AL.Methods1. Patients and controls:We selected 56 patients with AL, which divided into acute lymphoblatic leukemia (ALL) group and acute myeloid leukemia (AML) group a. And 20 healthy volunteers were studied as control group.2. We established real-time quantitative RT-PCR methods to detect the expression of miR-486 in patients with AL, and U6 was detected as internal control.3. The central tendency was responsed by the median M and variables were assessed by the Mann-Whitney U rank-sum test.Results1. The level of miR-486 in ALL group was significantly lower than that in AML group.2. The level of miR-486 between AML group and control group were not statistically significant.Conclusions1. The level of miR-486 was reduced in ALL patients indicating that miR-486 may take part in the pathogenesis of ALL2. There was no difference of the level of miR-486 between AML patients and control, indicating that miR-486 may have no obvious function on AML cells.