DcR3 Expression In Esophageal Squamous Cell Carcinoma And Its Correlation With Tumor Infiltrating T Lymphjocytes

Objective:To investigate the expression of decoy receptor3 (DcR3) and the distribution of T lymphocytes in the tissue of esophageal squamous cell carcinoma (ESCC), and to analyze its correlation with clinicopathological factors and the relationship between DcR3 and tumor infiltrating T lymphocytes (TIL), so that we could found its clinical significance and a new pass in gene treatment.Methods:Expression of DcR3 was detected in ESCC (n=60) and normal tissues (n=19) by SABC immunohistochemistry. CD45RO was used to measure the quantity and distribution of TIL. The relationship between the expression of DcR3 and age, gender, tumor size, histology, invasion depth, TNM stage, lymph node metastasis, pathological type and distribution of TIL was analyzed with concise statistical analysis software.Results:(1)The protein of DcR3 located mainly in the cell membrane and cytoplasm. It presented yellowish-brown in the experimental results. The DcR3 positive expression rate of 36.7%(22/60)in ESCC was higher than that in normal esophageal tissues(0 /19),χ2=9.656, P<0.05.(2)The relationship between DcR3 expression and clinicopathological factors in ESCC:Compared with the moderately and well differentiated ones 25.6%(10/39), it is higher that DcR3 protein positive expression rate of 57.1%(12/21) in poorly differentiated ESCC,χ2=5.833, P<0.05. The DcR3 positive expression rate of 51.3%(20/39)in the patients with tunica adventitia esophagi infiltration was significantly higher than that of 9.5%(2/21) in the patients without tunica adventitia esophagi infiltration,χ2=10.250, P<0.05. The DcR3 protein positive expression rate in clinical classification stageⅢandⅣESCC was 55.6%(15/27), significantly higher than that of 21.2%(7/33) in stageⅠandⅡ,χ2=7.542, P<0.05. The DcR3 gene expression was positively related to the tumor differentiation, infiltration depth and clinical, but not to the age, sex, tumor size, lymph nodes metastasis and pathological type.(3)TIL mainly distributed in interstitial among nests, but was rarely seen in tumor germinal center. The average of TIL was less in ESCC of DcR3 positive expression (18.51±6.02) than that in negative ones(22.57±5.15), t=2.769,P<0.05.Conclusion:(1) The over-expression of DcR3 was correlated with cell apoptosis and tumor genesis.(2) The DcR3 gene expression was obviously related to the tumor differentiation, infiltration depth and clinical, which has a relationship with tumor malignant behavior and invasive infiltrating ability.(3) The distribution of TIL predicts that there could be a correlation between DcR3 and immune supervision of T lymphocytes.(4) The abnormal expression of DcR3 may promote tumor genesis and progression of ESCC by blocking apoptosis and evading immune surveillance. The detection of DcR3 protein expression can be worthwhile to judge the malignance and progression of ESCC. There may be positive significance to interfere DcR3 expression.