Research On The Differtiation Of Bone Marrow Mesenchymal Stem Cells To Chondrocytes Induced By Acupuncture Combined Medicine

Objective:Observe the effect of acupuncture combined with medicine on rat bone marrow mesenchymal stem cell proliferation and GAG content,â…¡collagen expression, and investigate the effects of the Acupuncture blood serum combined the injection salviae miltiorrhiza on promoting the proliferation of bone marrow mesenchymal stem cells (BMSCs) and inducing to differentiate from BMSCs to chondrogenic cells phenotype.Methods:Acupunctured 20 SD rats with electroacupuncture for 7 days,then took a preparation of the common carotid artery blood serum. The BMSCs were separated from 30 SD rats aged 4 weeks bone marrow, which were cultivated in vitro, the third generation. BMSCs were randomly divided into 4 groups:control group, acupuncture blood serum group(acupuncture group), injection salviae miltiorrhiza group(drug group) and group of the acupuncture blood serum combined the injection salviae miltiorrhiza(combined group). BMSCs were induced to differentiate to chondrocytes by the different conditions.Results:Both the combined group and drug group could promote the proliferation of BMSCs(P<0.05). Particularly in the combined group, which were highly significant difference (P<0.01)compared with the control group, the effects were the most significant. There was no significant difference between the acupuncture group and the control group(P>0.05). Both the combined group and drug group were shown to promote the expression of collagenâ…¡(P<0.05). Particularly in the combined group, which were highly significant difference (P<0.01) compared with the control group, the effects were the most significant. There was no significant difference between the acupuncture group and the control group (P>0.05).Conclusion:The acupuncture blood serum combined the injection salviae miltiorrhiza can promote the BMSCs to proliferate and induce to differentiate from BMSCs to chondrogenic cells phenotype.