Effects Of Somatostatin Analogue Octreotide On Redox Status Of Mice Fed With High-fat

SS(somatostatin, SS)is a small regulatory peptide which has extensive inhibition effects on physiological function of gastrointestinal tract. This paper studies effects of SS analogue octreotide on digestion, absorption, metabolism and redox status of mice fed with high-fat diet, to clarify the regulation mechanism of body's redox status and provide theoretical basis to improve the health of the body.64 male C57BL/6 mice were divided into four groups: normal, high-fat, high-fat +octreotide(OCT), high-fat+0.1% lipoic acid (LA), 0.1ml OCT(20ug/kg) was injected in OCT group and metabolism experiment was implemented during the 3rd week, oral glucose tolerance test (OGTT) was done at the end of the 3rd week and then all the mice were executed, blood lipid index, anti-oxidation index of digestion, absorption and metabolism tissues, digestive enzyme levels of duodenum and jejunum were measured. Another 112 male C57BL/6 mice were divided as before, 0.1ml OCT(20ug/kg) was injected in OCT group during the 5th week, the high-fat group was divided into obesity, middle weight, obesity resistance group by weight at the end of the 5th week, half of the mice in each group were injected respectively 0.1ml OCT and NS, blood ROS and BG were measured after 10 minutes after injection, the next day all the mice were executed, blood lipid index, anti-oxidation index of digestion, absorption and metabolism tissues were measured, RT-PCR detection mRNA expression of SS and the IKKE in duodenum.Results show that: three-week high-fat diet can lead weight gain in mice, digestibility rate of crude protein, organic and dry material decrease in mice, levels of protease, amylase reduce, glucose tolerance impaired, lipid metabolic disorder, ROS and MDA levels increase, antioxidant enzyme levels reduce, OCT injection and LA addiction can reduce weight, enhance digestibility of crude protein, organic and dry material, improve the protease, amylase's levels, protect intestinal mucosa, alleviate glucose tolerance damaged state, improve blood lipid metabolism, improve antioxidant capacity of digestion, absorption and metabolism tissues in high-fat group; 5-week high-fat diet can lead obesity in mice, BG, blood ROS levels increase, lipid metabolism disorder, MDA increases, antioxidant enzyme activities reduce, expression of IKKE mRNA up-regulates, OCT injection can reduce weight in obesity mice, decrease BG, blood ROS levels, improve lipid metabolism, reduce MDA level and improve the body anti-oxidation ability, significantly raise SS mRNA expression, decrease IKKE mRNA expression, blood ROS positively correlate with BG and AI, significantly negatively correlate with HDL, SS and ROS, AI is a significant negative correlation.Conclusion: OCT injection can improve intestinal protease, amylase activities, improve nutrient digestibility rate, improve glucose tolerance impaired status and lipid metabolism disorder in mice feed with high-fat diet, reduce BG and blood ROS levels in obesity mice lead by high-fat diet, significantly raise SS mRNA expression, decrease IKKE mRNA expression, reduce body weight in obesity mice, decrease ROS and MDA levels in mice fed with high-fat diet and improve the body antioxidant capacity.