The Association Of ApoB And ApoAI Gene Polymorphism With Dyslipidemia In Han Chinese Of Xinjiang Shihezi

Objective:To explore the association of apolipoprotein B gene EcoRI and XbaI, apolipoprotein AI gene promoter region-75bp and intronⅠ+83bp polymorphisms and their haplotypes with dyslipidemia and plasma lipids index in Xinjiang Shihezi Han Chinese.Methods:Selecting 150 dyslipidemia patients and 150 peoples with normal plasma lipids of Xinjiang Shihezi Han Chineseas, conducted a case-control study. Apolipoprotein B gene EcoRI and XbaI and apolipoprotein AI gene promoter region-75bp and intronⅠ+83bp polymorphisms of all of the subjects were analyzed by PCR-restriction fragment length polymorphism (PCR-RFLP). Through comparing the distribution of frequencies of different genotypes and alleles of various sites and haplotypes of EcoRI and Xbal,-75bp and +83bp polymorphisms between dyslipidemia group and control group,the possible relationship between each sites polymorphism and dyslipidemia and plasma lipids index were analyzed.Results:(1)The frequencies of apolipoproteinB gene EcoRI and XbaI sites genotypes and alleles were different in dyslipidemia group compared to their controls(E+E-/E-E-genotype:37.33% vs 12.67%, P= 0.000;E-allele:19% vs 6.33%,P=0.000;X+X/X+X+genotype:20.66% vs 8%, P= 0.005;X+allele:11% vs4%, P=001). The frequencies of apolipoprotein AI gene -75bp and +83bp sites genotypes and alleles were not different in dyslipidemia group compared to their controls(P>0.05).(2)The levels of TC,TG, LDL-C and apoB of the E+E-/E-E-genetype and X+X-/X+X+ genetype were significantly higher than those of the E+E+ genetype and X-X- genetype in each of dyslipidemia group and control group(all P<0.05); The levels of HDL-C and apoAI of the M1+M1/M1-M1- genetype were significantly higher than those of the M1+M1+ genetype in each of dyslipidemia group and control group(all P<0.05). (3) Significant linkage disequilibrium was observed between apolipoprotein B gene EcoRI and XbaI and apolipoprotein AI gene-75bp and +83bp polymorphisms.(4)Only three haplotypes were found between apolipoprotein B gene EcoRI and XbaI polymorphisms:E+/X+,E+/X- and E-/X-, and the frequencies of haplotypes were different between dyslipidemia group and control group(χ2= 36.067, P=0.000);Only three haplotypes were found between apolipopro- tein AI gene -75bp and +83bp polymorphisms:M1+/M2+, M1+/M2- and M1-/M2+, and the frequencies of haplotypes were not different between dyslipidemia group and control group (χ2= 1.220, P=0.543).(5)The levels of TC,TG,LDL-C,apoAI and apoB of the dyslipidemia group were different among genotypic combinations of apolipoprotein B gene EcoRI and XbaI sites (P<0.05),the combination of E+E-/E-E- and X+X-/X+X+ genotype was associated with significantly higher level of TC; The levels of HDL-C,apoAI and apoB of the dyslipidemia group were different among genotypic combinations of apolipoproteinAI gene -75bp and +83bp sites(P<0.05), the combination of M1+M1-/M1-M1- and M2+M2+ genotype was associated with significantly higher level of HDL-C, the combination of M1+M1+ and M2+M2+ genotype was associated with significantly lower level of apoAI.Conclusions:(1)ApolipoproteinB gene EcoRI and Xbal polymorphisms maybe related to dyslipidemia in population of Xinjiang Shihezi Han Chinese, and the E- and X+Allele maybe the risk genetic factors for dyslipidemia. ApolipoproteinAI gene -75bp and +83bp polymoph- isms were not associated with dyslipidemia in population of Xinjiang Shihezi Han Chinese. (2)In population of Xinjiang Shihezi Han Chinese, there were very strong linkage disequilibrium between apolipoprotein B gene EcoRI and Xbal polymorphisms and between apolipoprotein AI gene -75bp and +83bp polymorphisms. (3)The haplotype between apolipoprotein B gene EcoRI and Xbal polymorphisms were associated with the risk of dyslipidemia, and the E+/X+ and E-/X- haplotype maybe the risk genetic factors for dyslipidemia. The haplotype between apolipoproteinAI gene -75bp and +83bp polymor- phisms do not increase the risk of dyslipidemia.