| Aims:To assess the protective effect of Ginkgo Biloba Leaves Injection on hepatic ischemia-reperfusion injury (HIRI) by established animal HIRI model and to provide clinical evidence.Methods:Fifty adult male Wistar rats which ranging in weight from 290g to 320g were divided at random into 5 groups:Sham operated group, control (HIRI) group, low dosing (30mg/kg) group, normal dosing group (60mg/kg) and high dosing (120mg/kg) group. All rats except sham operated group were dissociated and clamped the hepatic artery and portal vein with microvascular clamps after carried out laparotomy in order to achieve ischemia. After 30minutes ischemia, the clamps were opened and reperfusion was allowed for 120 minutes. Sham operated group were dissociated hepatoduodenal ligament only after given laparotomy. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) level, superoxide dismutase (SOD) activity and maleic dialdehyde (MDA) content were measured. Superoxide dismutase (SOD) activity, content of MDA and glutathione (GSH) in liver tissue were measured. Expression of Bcl-2 and Bax protein in hepatic tissue were detected with the immunohistochemistry methods.Results:Compared with control group:serum ALT and AST levels (P<0.01) and content of MDA (P<0.01) were significantly reduced and Activity of serum SOD (P<0.01) was increased in each dosing groups of Ginkgo-leaf; Also SOD (P<0.05, P<0.01) activity in liver tissue was significantly increased and content of MDA (P<0.01) and GSH (P<0.01) were significantly reduced in each dosing groups of Ginkgo-leaf; The expressions of Bcl-2 protein were significantly increased and the expressions of Bax protein were significantly reduced in each dosage groups of ASI in hepatic tissue (P<0.01). |
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