| Objective:1.To explore the effect of treadmill training in motor functional recovery in rats with spinal cord injury .2. To explain the relationships between motor recovery and CPG in incomplete spinal cord injury rat.Materials and Methods:Contusion spinal cord injuries (10g weight dropped 25 mm,n = 44 females,three-months SD rat) were performed on T10 using the NYU weight drop device. Then the injured rats were randomly divided into 2 groups: control (group A,n=12) and treadmill training groups(n=32). No treatment was performed on the control group, treadmill training was performed on the training group at 1 week after injury.After training for 6 weeks,the training group was randomly divided into 2 groups: group B1(n=12) and group B2(n=20),continue training was prepared for group B1 for 2 weeks,completely transection injuries were prepared for group B2 on T13.1 weeks after transaction, rats of group B2 were randomly divided into 2 groups: group C(control, n=10)and group D(training at 1 week after injury,n=10).Basso Beattie Bresnahan (BBB) scores and ACOS scores were performed weekly after operation for the control and step training groups,and at 7 days,10 days and 14 days for the transection groups.The expressions of serotonergic fiber were detected by haematoxylin-eosin staining and immunohistochemistry.Result:1. BBB scoresThe statistical difference of BBB score between group A and group B displayed at 2 weeks after operation (P < 0.05),and the BBB scores of group B were higher than that of group A and reach to 15.38±3.62 score after 8 weeks training.No statistical difference of BBB score displayed between group D and group C (P﹥0.05).2.ACOS scoresACOS scores showed the statistical difference between group A and group B at 2 weeks after operation (P < 0.05), it displayed a rapid recovery from 4 weeks to 6 weeks after training, then it seems to be smoothly.The ACOS scores of group B were reach to 78.00±10.39 which were higher than the score of group A.The statistical difference of ACOS score can be find between group D and group C (P< 0.05) 10 days after transaction . 3. Haematoxylin-Eosin stainingHaematoxylin-eosin staining showed the cavity formed and some glial cells in each group at 9 weeks after contusion injury, but both the area and the amount of glial cells of treadmill training group were smaller than that of the control and transaction groups.4. 5-HT immunohistochemistryThe immunohistochemistry staining indicated that the expression of 5-HT were more intense in the lumbar frontal horn caudal to the lesion in group B than that of group A (P < 0.05).Conclusion:1.Treadmill training can effectly promote motor functional recovery in rats with spinal cord injury.2.CPG plays a primary role in promoting motor functional recovery in rats with spinal cord injury.3.Treadmill training has a positive effect in plasticity of 5-HT fibers in the frontal horn caudal to the lesion.4. ACOS sore are more sensitive compared to BBB score in transaction rat. |
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