Chemical Constituents Of The Medicinal Fungus Phellinus Baumii

Phellinus baumii Pilat, a fungus belonging to the family Hymenochaetaceae, known as "Forest Gold", which is one of the most important Chinese traditional medicinal Phellinus fungi, preferably hosts on the stems of genus Prunus, Syringa, Crataegus, and other hardwood trees. Its fruiting body has been traditionally used for treatment of gastrointestinal cancer, liver or heart diseases, and stomach ailmentsa in China.In orther to investigate our Chinese precious Phellinus fungi resource, we systematically study the chemical composition of phellinus fungus. In this paper,57 compounds were isolated from the EtOAc portion of the 95% ethanolic extract of the fruit body of P. baumii and the artificial culture of phellinus fungus. Especially,50 compounds were isolated from natural phellinus baumii fungus,11 of which were new compounds, including two pairs of epimeric benzyl dihydroflavone and five hispidin-derived polyphenols, phellibaumins A-E, along with two new sterols. The structures of new compounds were elucidated by spectroscopic methods including IR, MS, HRMS, 1D and 2D NMR experiments. In addition, with help of the HPLC-CD coupling technique, the two new dihydroflavones were verified to be a mixture of two enantiomers respectively, and we found these epimeric pairs were non-equivalently present in nature. Inhibitory activities of phenolic compounds against NF-κB in a dose-dependent manner have also been tested. From the culture of phellinus,9 compounds were identified, all of which were isolated from this aritifical fungus for the first time.