American Ginseng Active Ingredients Do Insulin Resistance Fat Cells Mechanism Of Action Research Model

Insulin resistance (Insulin resistance, IR) is a certain amount of insulin the body is lower than expected biological response to the phenomenon of normal levels, that is the target organs of insulin (such as skeletal muscle, fat and liver) endogenous or exogenous insulin sensitivity and (or) reaction decreased, so that the normal amount of insulin the physiological effects of lower than normal, or more than the normal amount of insulin needed to achieve normal levels of physiological effects. Insulin resistance in non-insulin-dependent diabetes mellitus (NIDDM) or type 2 diabetes, the salient features, but also obesity, hypertension, abnormal lipid metabolism, atherosclerosis and other diseases common pathological mechanisms and the link between the [1] This study aimed to clarify the active components of ginseng cell model of insulin resistance in fat and mechanism, so as to further investigate the active components of American ginseng treatment mechanism of insulin resistance lay the foundation for American ginseng in diabetes, hypertension and insulin resistance in disease treatment experimental basis for the application.Objective:to study the imechanism of the active constituents of American Gins-eng impact the insulin resistance adipose cell model。Methods:They were extracted by ethanol and adsorbed by macro-reticular resins, then eluted with ethanol and isolated by silica gelc-olumn chromatography and HPLC preparation chromatography. Their st-ructures were determined by physical and chemical properties.as well as spectrographical identification.12 compounds were isolated fromthe active site the American ginseng impacts rat insulin resistance.Later,By Glucose consuming experiment,showed20 (R) ginsenoside Rbl,20 (R) ginsenosideRb-2,the Ginsenoside,pseudoginsenoside F11 may be the active constituents。the routine culture 3t3-L1 cells by inducement diferentiation to mature lipocite.administer contin dexamethasone and insulin compless nutrient solutionstimulate.to establish insulin resisitence modle.random to matched group,modelset americanginseng monomer ingrident four group,rosigitazone.after the drug interfere for 48 hours test the lever of TNF-α,Resistin,IL-6,FFA..Conclusions:1 Successfully build the insulin resistance adipose cell model.2 American ginseng monomer ingrident can reduce the concentration of TNF-α, Resistin,FFA,IL-6.show that thus reduce insulin resistance from the view of adipocytoki-nes.