The Related Factors Of Embryonic Outcome Of Human Oocytes Intercytoplasmic Sperm Injection

Human Assisted Reproductive Technology (ART) process in the treatment of infertility has made great progress and intracytoplasmis sperm injection (ICSI) as a treatment for less severe asthenozoospermia men the main measures has become an important assisted reproductive technology. The most important factors that affect the outcome of ICSI is the quality of ovum, we can evaluate it by two aspects: cytoplasmic development and nuclear growth. Mainly the degree of cytoplasmic development can be evaluated through the state of the contents of cytoplasm. The level of nuclear development can be evaluated it through the emergence of the first polar body. In the ICSI process, we can through the color of cytoplasmic particlesm, size of the vacuoles, Vacuole, inclusion body, inclusion body, first polar body morphology and so on to judge the quality of ovum and to guide the selection of implantation embryo for the outcome of the improvement of ICSI.Objective1,To understand the relationship of different types of first polar body of human oocytes and normal fertilization rate, cleavage rate and high quality embryo rate after ICSI using three sources of sperm 2,To understand the relationship of the state of cytoplasm of human oocytes and normal fertilization rate cleavage rate and high quality embryo rate after ICSI.MethodsMethod of retrospective study that 1017 MⅡof human oocytes in 110 cycles of ICSI from February 2009 to August 2009.①the oocytes were divided into three groups by the sources of different Semen:masturbation sperm retrieval group, PESA/TESA sperm retrieval group, frozen semen group;②First polar body morphology were divided into four types;③According to the shape of eggs were divided into two groups:the group one is normal eggs and another is abnormal.④Oocytes by the presence of a special form within the structure is divided into two groups:no special structure egg group and containing the egg cavity, refractive body, smooth endoplasmic reticulum (SER) aggregates of small sacs, the yolk membrane abnormalities, abnormal egg cell gap egg group:1,The relationship of four types of oocytes first polar body from three different groups of semen after ICSI normal fertilization rate after cleavage rate and high quality embryo rate.2,The relationship between group of normal egg and group of abnormal egg normal fertilization rate, cleavage rate and high quality embryo rate after ICSI.3,The relationship between group of no special structure egg group and containing the egg cavity, refractive body, smooth endoplasmic reticulum (SER) aggregates of small sacs, the yolk membrane abnormalities, abnormal egg cell gap egg group after ICSI normal fertilization rate after cleavage rate and high quality embryo rate.Statistical Package for all data handled by SPSS 16.0. Count data byχ2 test. Test for theα=0.05.Results1,There was significance comparing respectively normal fertilization rate with polar body typeⅢof oocytes {63.87%(244/382)} and polar body typeⅠof oocytes {76.33%(158/207)} in retrieval masturbation sperm (χ2=9.610, P< 0.05) 2,There was significance comparing respectively normal fertilization rate with Type the full rules oocytes first polar body rule-based group {70.80%(337/476)} and non-intact oocytes first polar body group {64.70%(350/541)} (χ2=4.303, P <0.05)3,There was significance comparing respectively normal fertilization rate with non-special structure eggs group {71.93% (551/766)} and the eggs with the special structure group {54.18%(136/251)} (χ2=27.170, P<0.05)4,There was significance comparing respectively normal fertilization rate with non-special structure eggs group {71.93% (551/766)} and the abnomal intercellular spaces egg group {50%(14/28)},abnormal vacuolar egg group {44.44%(8/18)},abnormal yolk membrane egg group {38.38%(38/99)} (χ2=6.331, P<0.05; X2=6.494, P<0.05;χ2=45.415, P<0.05)5,There was significance comparing respectively the third day of the quality embryo rate with non-special structure eggs group {59.54%(312/524)} and the eggs with the special structure group {44.27%(58/131)} (χ2=9.938, P<0.05)6,There was significance comparing respectively the third day of the quality embryo rate with non-special structure eggs group{59.54%(312/524)} and abnormal yolk membrane egg group {28.57%(10/35)} (χ2=14.942, P<0.05)Conclusion1,Complete the first polar body and the rules of the oocytes by ICSI had better fertilization.2,Normal form oocytes after ICSI fertilization can be done better.3,Yolk membrane tension is important structure to maintain the vitality of embryos, the yolk membrane tension difference led a direct result:degradation of embryos or dysplasia.