The Application Of Fluoreseence In Situ Hybridzation In The Diagnosis Of Bladder Carcinoma

Objective:This study is to discuss the method of fluorescence in situ hybridization in bladder cancer diagnosis, by comparing the CT, cystoscopy, urine cytology three methods of specificity, sensitivity, and the pathological stage and were compared with clinical classification. FISH method as a bladder cancer specific auxiliary examination methods applied significance. Methods:from November 2010 to February 2011 between the collection of Urology, First Affiliated Hospital of Xinjiang Medical University of suspicious urothelial tumors and bladder cancer patients after treatment of 80 cases reviewed in the morning urine.80 cases into 61 cases of male patients,19 females (aged 32 to 81 years, mean 61±11.45 years). Specimens from 10 healthy adult volunteers in the morning urine as the control group, of which 5 males and 5 females (aged 21 to 67 years, mean 51±8.14 years) for the establishment of the normal FISH detection threshold. Concurrent CT, cystoscopy, urine cytology, and in accordance with pathological TNM classification and clinical staging were compared. Results:FISH examination of sensitivity than the other three checks, the specificity of urinary cytology was flat, higher than the CT examination and cystoscopy. By ROC curve:FISH diagnosis of the maximum reference value. FISH area under the curve for the four largest diagnostic methods, indicating that the sensitivity and specificity to consider comprehensive FISH detection method is better than the other three methods. In different pathological grade and stage of the comparison. Higher sensitivity of FISH for low-grade tumors that are more sensitive. For TNM clinical stage. FISH sensitivity is high, found in high stage tumors more likely. IV diagnosis of pathological grade and in different clinical stages found in the comparison. FISH in Pathology sensitivity of low-grade group was significantly higher than the other three methods; in the pathology of high grade group, the inspection sensitivity than urinary cytology, cystoscopy, with the sensitivity of CT was flat, indicating that the low score FISH check the sensitivity of tumor grade was significantly better than the current method of examination in the high-grade tumors also have a distinct advantage. One of the group in Ta-T1 sensitivity of FISH was significantly higher than the other three methods; in the T2-T3 stage group, the inspection sensitivity than urinary cytology, cystoscopy, with the sensitivity of CT was flat, indicating the low stage FISH check the sensitivity of the tumor was significantly better than the current inspection methods, and also in high-stage tumors have a distinct advantage. Conclusion:FISH technique is a very effective non-invasive examination methods, with a high degree of sensitivity and specificity, but also for tumor grade and stage have a very good guide, good maneuverability, high stability, can be very useful adjunct to the diagnosis of bladder cancer.