A Study On Genes Associated With Bone Metastasis Of Breast Cancer In Xin Jiang Uygurs

Objective:Preliminary investigate genes genes associated with breast cancer bone metastasis in Xinjiang Uygurs,and find out a gene-expression signeture to predict early bone metastasis of breast cancer, then lay the groundwork for further molecular mechanism study of bone metastasis. Methods:The synchronous Bone Metastasis,their matched Breast Cancer tissue from 4 XinJiang Uygur patients were used for double-colored oligonucleotide microarrays assay and We selected differentially expressed genes with fold-change 2.0 or higher (logarithm base 2≥1-fold) in each microarray data for further analysis.Bioinformatics tools,such as,mas 3.0,Chilibot, Toppgene and Endeavor were used in turn for gene functional analysis, kegg pathway analysis,literature ananotaions,genes similarity function analysis. Results:We selected 154 differentially expressed genes overlapped in the four Bone Metastasis tumors/primary Breast Cancer groups,89 up-regulated and 66 genes were downregulated. Literature-based annotation and genes similarity function analysis with Chilibot, Toppgene and Endeavor showed that the 154 differentially expressed genes significantly enriched 44 known Bone Metastasis-related genes and 110 unknow metastasis-related genes,there are 20 genes among the 110 might have the similar function as bone metastasis.we found these 44 and 20 genes differentially expressed in different groups,but shared in a gene-correlation network.Combined with kegg pathway analysis, we found differentially expressed genes of the Bone Metastatic tumors participate in enriched pathways (p<0.05) involved in Cancer metastasis,Metabolism,Immune System and et al. such as,Glycolysis,cell adhesion,cell cycle regulation pathways,et al. Conclusion:Compared with breast cancer tissue, The gene expressfiles of bone metassis cancer is similar,64 genes of the 154 differentially expressed genes may be related to Bone Metastasis in Xinjiang Uygur women with invasive ductal cancer;and found Bone Metastatic tumors shared a number of pathways associated with metastasis,such as Metabolic pathways,Immune system,Cell adhesion, et al.These may serve as potential prognostic markers for prediction and novel targets for therapeutic intervention in bone metastasis of Uygur breast cancer patients.