The Study Of Application HPLC-DAD & HPLC-MS On The Detection Of Carcinogenic Dyes On Textiles

This paper focused on the study of nine carcinogenic dyes which might be present on the textiles, namely, C.I. Acid Red 26, C.I. Disperse Orange 11, C.I. Disperse Yellow 3, C.I. Disperse Blue 1, C.I. Direct Blue 6, C.I. Direct Black 38, C.I. Basic Red 9, C.I. Basic Violet 14. During the research, we have studied their optimizing stripping process and proposed the use of high-performance liquid chromatography-diode array detection (HPLC-DAD) and liquid chromatography-tandem mass spectrometry (HPLC-MSD) methods for their detection, which provides a convenient means of pre-treatment and testing methods for the detection of carcinogenic dyes on textiles.A convenient and efficient stripping methods was proposed by analyzing the stripping conditions, such as stripping agent, ultrasonic power, ultrasonic time and temperature, pH and additives. The results indicated that an ideal stripping effect will be obtained for the nine carcinogenic dyed textiles by the choice of methanol coupled with 15%(W/V) urea as the extraction agent, and applying 100 W of ultrasonic cleaning machine treatment for 30 min under 70℃and certain pH conditions.A high performance liquid chromatography-Diode array Detection (HPLC-DAD) method was proposed here to offer a sensitive approach for efficient identification of nine carcinogenic azo dyes in one-step. The central composite design (CCD) was employed to find the optimal chromatographic parameters. Chromatography separations were performed by using a linear gradient method with the 2.5 mmol/L dihexylamnonium acetate and 2.5 mmol/L sodium 1-heptanesulfonate buffer-acetonitrile mobile phase, the separation column used was the TopsilTM 5μm Ci8 and the results were detected by diode array detection. Results of chromatogram demonstrate that the proposed method is able to obtain peaks that are evenly distributed through the chromatogram and prevent peak overlap. The calibration curves showed good linearity in the range of 1.0-100 mg/L levels with the correlation coefficients above 0.99 respectively and the limits of detection (LOD) and quantification (LOQ) of the method are in the ranges of 0.62～20.87 mg/kg,2.05～68.87 mg/kg, respectively.A high performance liquid chromatography-tandem mass spectrometry (HPLC-MSD) method was developed for the determination of nine carcinogenic dyes. The separation of nine carcinogenic dyes obtained previously was performed on a Varian pursuit 3 C18 column by applying a gradient elution with 5 mmol/L ammonium acetate solution and acetonitrile, and the results were detected with different ionization mode. The calibration curves showed good linearity in the range of 12.5-1000μg/L levels with the correlation coefficients above 0.95 respectively. The limits of detection and quantification were 0.060～0.44μg/kg and 0.20～1.5μg/kg respectively, which is sufficient for the detection of carcinogenic dyes on fabrics.