| Purpose:Exploration on pathogenic mechanism of kidney-Qi deficiency and phlegm stasis in Alzheimer's disease by establishing the muti-factor compound rat model of Alzheimer's disease(AD), to explore the interference and therapeutic mechanism of Buyuan Congnao Decoction (BCD) in treating AD from behaviology and nerve cells apoptosis.method:To adopt IBO and Aβ1-42 of the mixed solution inject to sea horse area establish AD rat models. Carrying on Chinese medicine intervention by BCD, and taking Piracetam-NEGPF as to be the checking-medicine. And apply Morris water maze method to examine memory abilities of study on initiative and passive on AD rat models. Observed pathological morphology of hippocampus by the light microscope. The expression of apoptosis related protein Bax and Bcl-2 in rats'hippocampus were studied by immunohistochemistry method.Results:1. Ethology: compared with normal group, latency period of escape in model group prolonged obviously, frequency of finding the platform decreased, number of times of mistake increased(P<0.01). Treat group compared with model group, latency period of escape shortened, frequency of finding the platform increased, number of times of mistake decreased (P<0.01). Regardless of Chinese medicine and Western medicine, both of them are available. And the treatment of Chinese medicine and Western medicine contains close, to say in other words, they are no distinguish differences(P>0.05).2. Apoptosis: compared with normal group, protein level of bax ascended, bcl-2 descended (P<0.01). Treat group compared with model group, protein level of bax descended, and bcl-2 ascended (P<0.01). no matter what Chinese medicine and Western medicine are no conspicuous differences (P>0.05). 3. Morphology change: number of neuron of model group was less than that of normal group, and sum of pathologic neuron was more than that of normal group. Cell apoptosis phenomenon could be seen, such as cell nucleus of some neuron showed pyknosis condition, caryotheca thicked and aberration, degeneration, necrosis, lipofuscin increased and so on. Both of BCD treat group and checking-medcine can decreased number of pathologic neuron, Compared with model group, distribution of neurofibra was more regular.Conclusion:1. Kidney deficiency and phlegm stasis is the basic etiological factor for AD, and replenishing kidney and removing phlegm stasis therapy is active on treating AD.2. The animal model of Alzheimer's disease induced by composite Aβ1-42 and IBO injected into basal nucleus of Meynert of rats simulated some characteristics and pathology of human AD to some extent, which may used as a reliable model to study AD.3. Buyuan Congnao Decoction (BCD) could decrease the expression of bax of hippocampus neural cells of rats, and increase the expression of bcl-2, and suppress the apoptosis of hippocampus neural cells of rats.4. The protection function of Buyuan Congnao Decoction (BCD) which to the AD rats'brain nerve cells is possible that strengthen expression to damaged hippocampus neuron bcl-2, and decrease to bax .Thus the study and memory of the AD rat is improved. |
PDF Full Text Request