Mongolian Medicine A Research, The Effect And Mechanism Of Carbon Hemostasis

Objectives:We study the hemostatic effects of carbonized Agei and explore the hemostatic mechanism. These provide experimental basis for its clinical application.Methods:1. The mouse bleeding time was measured by severing tails test, and the clotting time was measured by capillary test.2. The experimental model of gastric ulcer was established by Indomethacin and absolute ethyl alcohol. Observing the changing of gastric ulcer exponential after prophylactic, we analyze the effects of carbonized Agei on the mouse gastric ulcer exponential.3. The PT, APTT, TT and FIB were measured for analyze the effects of carbonized Agei on coagulation system.4. The MFI of CD61 and the positive rate of CD62P were measured by Flow Cytometry. We analyze the effects of carbonized Agei on platelet activation system. Combined with the changing of platelet count and morphology, we ananlyze the effects of carbonized Agei on platelet system.5. The indexes of hemorrheology were measured by blood rheometer. We analyze the risk of carbonized Agei on thrombosis. Results:1. We obtain effects of different extract from carbonized Agei on the mouse BT and CT. In the group with carbonized Agei, BT and CT were significantly declined (P<0.01). The water part and n-butanol extracted from carbonized Agei can significantly shorten mouse BT and CT (P<0.01). The ethyl acetate extracted from carbonized Agei had no effect on mouse BT and CT (P>0.05)We obtain effects of different extract from carbonized Agei on the heparinized mouse BT and CT. In the group with carbonized Agei, BT and CT were significantly declined (P<0.01). The water part, n-butanol and ethyl acetate extracted from carbonized Agei can shorten heparinized BT and CT (P<0.01或P<0.05)2. The low dose group, middle dose group and high dose group of carbonized Agei can significantly shorten mouse gastric ulcer exponential (P<0.01).3. The middle dose group and high dose group of carbonized Agei can shorten mouse PT (P<0.01或P<0.05). The high dose group of carbonized Agei can shorten mouse APTT(P<0.05). The low dose group, middle dose group and high dose group of carbonized Agei had no effect on mouse TT and FIB (P>0.05)4. The high dose group of carbonized Agei can significantly increase the MFI of CD61and the positive rate of CD62P (P<0.01) 5. The middle dose group and high dose group of carbonized Agei can significantly increase the platelet count(P<0.01). The low dose group, middle dose group and high dose group of carbonized Agei can significantly shorten platelet distribution width (P<0.01). It had no effect on the mean corpuscular volume (P>0.05)6. The low dose group of carbonized Agei can increase the erythrocyte deformability of red cell (P<0.05). The middle dose group of carbonized Agei can decrease blood sedimentation (P<0.05). The high dose group of carbonized Agei can shortenr cell morphology (P<0.05) The low dose group, middle dose group and high dose group of carbonized Agei had no effect on the whole blood viscosity of rabbit in different shear rate, plasma viscosity, blood reduction viscosity, the gathering index, erythrocyte index of rigidity of red cell (P>0.05)Conclusions:1. The carbonized Agei, the water part and n-butanol extracted from carbonized Agei have the favorable effects on haemostasis and coagulation, but carbonized Agei as a whole has better effect according to its developing trend.2. Carbonized Agei has favorable effects on preventing and curing the experimental gastric ulcer model.3. Carbonized Agei can perform function of hemostasis through intrinsic and extrinsic coagulation pathway. 4. Carbonized Agei can perform function of hemostasis through increased platelet count and platelet activation.5. Carbonized Agei can reduce the peripheral resistance of blood vessel and inhibit thrombosis to a certain degree through decrease blood sedimentation and increase the erythrocyte deformability of Red Cells.