Study Of The Chemical Composition Of Traditional Chinese Medicine Pulsatilla

Objective: To study the chemical constituents of Pulsatilla chinensis.Method: The constituents were isolated and purified by various chromatographic techniques and were elucidated by chemical and spectral methods.Result: Eleven triterpenoid saponins were isolated from the root of Pulsatilla chinensis. The structures were identified as oleanolic acid (Ⅰ), hederagenin (Ⅱ), oleanolic acid 3-O -β-D-glucopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-α-L-arabinopyranoside (Ⅲ), oleanolic acid 3-O-β-D-glucopyranosyl-(1→4)-β-D-glucopyranosyl-(1→3)-α-L- rhamnopyranosyl-(1→2)-α-L-arabinopyranoside (Ⅳ), oleanolic acid 3-O-α-L-rhamno pyranosyl-(1→2)-α-L-arabinopyranoside (Ⅴ), 3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-rhamnopyransyl-(1→4)-β-D-gluco pyranosyl-(1→6)-β-D-glucopyranosyl ester (Ⅵ), 3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl lup-20(29)-en-28-oic acid 28-O-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (Ⅶ), 3-O-α-L-rhamnopyranosyl- (1→2)-α-L-arabinopyranosyl hederagenin 28-O-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (Ⅷ), hederagenin 3-O-β-D- glucopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-α-L-arabinopyranoside (Ⅸ), 3-O-β-D -glucopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-α-L-arabinopyranosyl hederagenin 28-O-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester(Ⅹ), 3-O-β-D-glucopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosylhederagenin 28-O-α-L-rhamnopyransyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (Ⅺ).Conclusion: Three types of saponins including oleanolic acid, hederagenin and betulic acid were isolated. And theses compounds have a certain regularity in the structure,which can be used for structure-activity relationship study.