| Objective: By observing the pre-clinical and animal experiments and effective anti-fibrosis prescription Qigongkangxian Kangxian Fang (QF) of the demolition of prescription drugs Endometriosis (centipede, mountain arrowhead) on liver function and liver fibrosis, liver histopathology; and QF in Endometriosis by observing the drug (centipede, mountain arrowhead) on the transforming growth factor (TGFβ1) and Smads protein in the regulation of expression, to further explore the Empirical Study of the mechanism of hepatic fibrosis.Method: The SD rats were randomly divided into four groups of 10 each.①②model control group control group③Endometriosis drug dose in the intervention group④Endometriosis drug dose in the intervention group. In addition to the normal control group received normal saline (0.5ml / 2 times / week) by intraperitoneal injection, the other groups were given pig serum 0.5ml / intraperitoneal injection 2 times per week, were injected 8 weeks to induce hepatic fibrosis model. Fifth week,①,②the two groups with normal saline 2ml/100g, 1 time / day,③,④the size of the two groups fed with oral doses of Endometriosis drugs (crude drug content in 1 g / ml), Endometriosis low-dose intervention group Endometriosis granules 1ml/200g administered services, 1 time / day; Endometriosis Endometriosis dose group fed granule 2ml/200g services, 1 time / day, four weeks of the experiment 8 weeks. 8 weeks after the preparation of the eye blood serum reserve; off neck rats were killed, taken as part of the left lobe of liver tissue in 10% formalin fixed, paraffin sections to prepare for HE, Mallory, immunohistochemistry; light microscopy of liver cells change and the degree of liver fibrosis; immune staining of liver tissue TGF-β1 and smads related protein expression; automatic biochemical analyzer serum indicators of liver function (ALT, A, G, A / G) and ELISA Miriam ABC-ELISA method to measure the levels of serum TGF-β1.Result:1, with model group, Endometriosis drug dose in the intervention group decreased serum ALT (P <0.05), A / G value increased. 2, HE staining results showed that: After treatment, the liver compared with model group, treatment group, liver damage, liver cell edema were lighter than the model group, the overall situation has obviously improved. Endometriosis high dose group, the intervention group performance of liver regeneration, liver fiber to improve the situation of low dose treatment group than in Endometriosis obvious. 3, model Mallory staining of liver tissue biopsy of liver fibrosis. 4,Immunohistochemistry showed that: the normal group only weak expression of rat liver TGFβ1, Smad2, Smad3, the model group than normal group TGFβ1, Smad2, Smad3-positive signals increased significantly (P <0.01), Endometriosis rat liver after drug intervention TGFβ1 expression was significantly lower than the model, while Smad2, Smad3 protein expression decreased, large and small dose group compared with the model are different. 5, TGF-β1 levels: model group, Endometriosis drug intervention in the TGFβl serum significantly reduced (P <0.01)Conclusion: Qigongkangxian Kangxian Fang (QF) in Endometriosis drug on the immune injury of rat liver fibrosis and its reversal, but on promoting liver fibrosis cytokine TGF-Smad protein pathway has a good regulation, the therapy Liver fibrosis has some significance. |
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